This azadibenzocyclooctyne-tetramethyl rhodamine (TAMRA) derivative is a versatile fluorescent reagent for labeling azide containing molecules or compounds. Cyclooctynes are useful in strain-promoted copper-free azide-alkyne click chemistry reactions.This dibenzocyclooctyne will react with azide-functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage. Subsequent labeled molecules can be visualized by fluorescence spectroscopy.
ACS chemical biology, 7(8), 1326-1330 (2012-06-14)
Transcriptional activity from a specified promoter can provide a useful marker for the physiological state of a cell. Here we introduce a method for selective tagging of proteins made in cells in which specified promoters are active. Tagged proteins can
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