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SRE0006

Sigma-Aldrich

Thymidine Phosphorylase, recombinant from Escherichia coli

recombinant, expressed in E. coli, Suitable for manufacturing of diagnostic kits and reagents, buffered aqueous solution, ≥500 units/mL

Synonym(s):

Gliostatins, PD-ECGF, Thymidine:orthophosphate deoxy-D-ribosyltransferase

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

form

buffered aqueous solution

concentration

≥500 units/mL

technique(s)

inhibition assay: suitable

color

colorless to yellow

solubility

soluble
water: soluble

NCBI accession no.

UniProt accession no.

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

2-8°C

Gene Information

Escherichia coli ... deoA(948901)

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General description

Research area: CELL SIGNALING

The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which is identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.

Application

Thymidine phosphorylase has been used:
  • in a study to evaluate biomarkers for advanced breast cancer patients treated with capecitabine-based first-line chemotherapy.
  • in a study to investigate implications for the clinical efficacy of nucleoside analogues.

Biochem/physiol Actions

An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which has been found to be identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.
Thymidine phosphorylase catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the thymidine salvage pathway and pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. The enzyme inhibits apoptosis and induces angiogenesis thereby promoting tumor growth and metastatic process. Moreover, thymidine phosphorylase inhibits vascular smooth muscle cell proliferation.

Unit Definition

One unit will convert 1.0 μmole each of thymidine and phosphate to thymine and 2-deoxyribose 1-phosphate per min at pH 7.4 at 25°C.

Preparation Note

Cloned from E. coli and produced in overexpressing E. coli

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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The dual role of thymidine phosphorylase in cancer development and chemotherapy
Bronckaers A, et al.
Medicinal Research Reviews, 29(6), 903-953 (2009)
Thymidine kinase 1 and thymidine phosphorylase expression in non-small-cell lung carcinoma in relation to angiogenesis and proliferation
Brockenbrough J S, et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 57(11), 1087-1097 (2009)
Structures of native human thymidine phosphorylase and in complex with 5-iodouracil
Mitsiki E, et al.
Biochemical and Biophysical Research Communications, 386(4), 666-670 (2009)
Hriday Bera et al.
European journal of medicinal chemistry, 67, 325-334 (2013-07-23)
Thirty-three 1,2,4-triazolo[1,5-a][1,3,5]triazin-5,7-dione and its 5-thioxo analogues were designed and synthesized which contained different substituents at meta- and/or para-positions of 2-phenyl or 2-benzyl ring attached to the fused ring structure. The preliminary pharmacological evaluation demonstrated that the 5-thioxo analogues of 1,2,4-triazolo[1,5-a][1,3,5]triazine
Jen-Chung Ko et al.
Biochemical pharmacology, 88(1), 119-127 (2014-01-23)
Tamoxifen is a triphenylethylene nonsteroidal estrogen receptor (ER) antagonist used worldwide as an adjuvant hormone therapeutic agent in the treatment of breast cancer. However, the molecular mechanism of tamoxifen-induced cytotoxicity in non-small cell lung cancer (NSCLC) cells has not been

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