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Key Documents

S7531

Sigma-Aldrich

Anti-Staphylococcal α-Toxin antibody produced in rabbit

whole antiserum

Synonym(s):

Anti-α-Hemolysin

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

mol wt

antigen 33 kDa

contains

15 mM sodium azide

species reactivity

Staphylococcus aureus

packaging

antibody small pack of 25 μL

technique(s)

dot blot: 1:20,000
indirect ELISA: 1:50,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Staphylococcal α toxin, a water soluble 33kD single polypeptide, is a cytotoxic agent secreted by Staphylococcus aureus. This protein has membrane damaging properties and accounts for erythrocyte lysis. Hence it is also referred to as lethal hemolytic toxin or α-hemolysin . Anti-staphylococcal α-toxin antibody can be used for studies of the toxin-membrane interaction. Rabbit anti-staphylococcal α-toxin antibody reacts specifically with staphylococcal α-toxin but not with staphylococcal enterotoxin A, cholera toxin or pseudomonas exotoxin A.

Immunogen

α-toxin (α-hemolysin) from Staphylococcus aureus

Application

Anti-staphylococcal α-toxin antibody can be used in indirect ELISA (1:50,000) and dot blot immunoassay (1: 20,000).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)

Quality

The antiserum has been treated to remove lipoproteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S Forti et al.
European journal of biochemistry, 181(3), 767-773 (1989-05-15)
alpha-Toxin, a lethal hemolytic toxin secreted by Staphylococcus aureus, forms ionic channels of large size in lipid membranes. To investigate the mechanism of channel assembly we have studied the kinetics of pore formation on small unilamellar vesicles. We have used
Alicia Lacoma et al.
Toxins, 13(2) (2021-02-11)
Staphylococcus aureus is a commensal and frequent colonizer of the upper respiratory tract. When mechanical ventilation disrupts natural defenses, S. aureus is frequently isolated from the lower airways, but distinguishing between colonization and infection is difficult. The objectives of this
Olivia A Todd et al.
mBio, 10(3) (2019-06-06)
Candida albicans and Staphylococcus aureus are among the most prevalent nosocomial pathogens that are responsible for severe morbidity and mortality, even with appropriate treatment. Using a murine model of polymicrobial intra-abdominal infection (IAI), we have previously shown that coinfection with
Shi-Jie Wan et al.
Frontiers in chemistry, 10, 877469-877469 (2022-04-19)
The emergence of antibiotic resistance in Staphylococcus aureus has necessitated the development of innovative anti-infective agents acting on novel targets. Alpha-hemolysin (Hla), a key virulence factor of S. aureus, is known to cause various cell damage and death. In this
María Belén Mestre et al.
Autophagy, 6(1), 110-125 (2010-01-30)
Staphylococcus aureus is a pathogen that causes serious infectious diseases eventually leading to septic and toxic shock. Classically S. aureus has been considered an extracellular pathogen, but cumulative evidence indicates that it invades cells and replicates intracellularly leading to staphylococcal

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