This product is recommended for ELISA (microwell) procedures and is not recommended for membrane applications.
N7653
p-Nitrophenyl Phosphate Liquid Substrate System
alkaline phosphatase substrate, liquid
Synonym(s):
4-Nitrophenyl phosphate disodium salt solution, pNPP
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About This Item
Linear Formula:
O2NC6H4OP(O)(ONa)2
CAS Number:
Molecular Weight:
263.05
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
MDL number:
Pricing and availability is not currently available.
Product Name
p-Nitrophenyl Phosphate Liquid Substrate System, liquid
form
liquid
Quality Segment
shipped in
wet ice
storage temp.
−20°C
SMILES string
[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
InChI
1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
InChI key
VIYFPAMJCJLZKD-UHFFFAOYSA-L
General description
The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.
Application
p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase in alkaline phosphatase assay.
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
Biochem/physiol Actions
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol. It is a common chromogenic substrate for alkaline phosphatase.
Physical form
Ready-to-use.
Disclaimer
Stable at least one year when stored below 0 °C.
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This Item | |||
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| description liquid | description p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate, CAS 4264-83-9, is an excellent substrate for alkaline phosphatase-based ELISA assays. Produces yellow soluble end product (405-410 nM). | description ready to use solution | description alkaline phosphatase substrate |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| form liquid | form crystalline solid | form liquid | form liquid |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in ambient | shipped in - | shipped in - |
signalword
Danger
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral
target_organs
Kidney,Liver,Blood
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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What type of application can Product N7653, p-Nitrophenyl Phosphate Liquid Substrate System, be used in?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How should Product N7653, p-Nitrophenyl Phosphate Liquid Substrate System, be used?
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Information on usage is found in our product information sheet (under Documents, above).
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What is the concentration of Product N7653, p-Nitrophenyl Phosphate Liquid Substrate System?
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The concentration of this product is considered proprietary.
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