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Neutral Red Solution (0.33%)

Name: Neutral Red Solution (0.33%)
Brand: SIGMA

3.3 g/L in DPBS, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

Neutral Red solution, 3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Toluylene red

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About This Item

Empirical Formula (Hill Notation):

C₁₅H₁₇ClN₄

CAS Number:

553-24-2

Molecular Weight:

288.78

NACRES:

NA.75

PubChem Substance ID:

24897603

UNSPSC Code:

12352207

MDL number:

MFCD00012651

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Properties

sterility

sterile-filtered

Quality Segment

400

product line

BioReagent

form

solution

concentration

3.3 g/L in DPBS

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

SMILES string

[Cl-].Cc1cc2N=C3C=C\C(C=C3Nc2cc1N)=[N+](/C)C

InChI

1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H

InChI key

PGSADBUBUOPOJS-UHFFFAOYSA-N

Description

General description

Neutral Red is a cationic dye that is used for nuclear counterstaining in biological research. It also serves as a pH indicator in analytical laboratories. On decomposition, neutral red dye releases carbon monoxide (CO), carbon dioxide (CO₂), nitrogen oxides, and hydrogen chlorides. Therefore, this dye is considered toxic and is recommended to remove before discharge into receiving streams.

Application

Neutral Red Solution (0.33%) has been to stain viable cells and measure cell viability. It has also been used as a component of culture medium for enzymatic digestion of ovarian medulla tissue to extract follicles.

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This Item	N6264	N7005	861251
Sigma-Aldrich N2889 Neutral Red Solution (0.33%)	Sigma-Aldrich N6264 Neutral Red Solution, Buffered	Sigma-Aldrich N7005 Neutral Red	Sigma-Aldrich 861251 Neutral Red
description 3.3 g/L in DPBS, sterile-filtered, BioReagent, suitable for cell culture	description -	description Dye content ≥90 %	description certified by the BSC
technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) titration: suitable	technique(s) titration: suitable
concentration 3.3 g/L in DPBS	concentration 0.5% (w/v, acetate buffer, pH 5.2. Preservative added.)	concentration -	concentration -
form solution	form solution	form powder	form powder
Quality Level 400	Quality Level 500	Quality Level 200	Quality Level 100
storage temp. 2-8°C	storage temp. room temp	storage temp. 2-8°C	storage temp. room temp
product line BioReagent	product line -	product line -	product line -

Storage Class

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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