M7403

MCDB 153 Medium

With trace elements, L-glutamine and 28mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): Molecular, Cellular, and Development Biology 153 medium
• NACRES: NA.75
• UNSPSC Code: 12352207

Properties

• Quality Segment: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: NaHCO₃: no; L-glutamine: 0.8772 g/L; glucose: 1.081 g/L (Dextro); sodium pyruvate: 0.055 g/L; phenol red: 0.001242 g/L; HEPES: 6.6 g/L (25mM)
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

MCDB 153 media are modifications of Ham′s nutrient mixture F-12, designed for the growth of human keratinocytes, clonal growth of chicken embryo fibroblasts, and Chinese hamster ovary (CHO) cells using low levels of fetal bovine serum protein (FBSP), extensive trace elements or no serum protein.

Application

MCDB 153 Medium has been used as a component of melanoma isolation media (MIM) to culture human non-metastatic and metastatic melanoma cells.

Preparation Note

Formulated to contain 17.6 grams of powder per liter of medium.

Supplement with 1.18 g/L sodium bicarbonate.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable