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Medium 199

Name: Medium 199
Brand: SIGMA

With Earle′s salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Cell Culture Medium, M199 Medium, Medium 199 Solution, TCM 199

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.71

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Properties

Product Name

Medium 199, With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Quality Segment

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: yes
phenol red: yes
NaHCO₃: no
sodium pyruvate: no
Earle’s salts (5% CO₂): yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

Description

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Preparation Note

Formulated to contain 9.5 grams of powder per liter of medium.

Supplement with 2.2 g/L sodium bicarbonate.

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This Item	M7528	M2520	M0650
Sigma-Aldrich M5017 Medium 199	Sigma-Aldrich M7528 Medium 199	Sigma-Aldrich M2520 Medium 199	Sigma-Aldrich M0650 Medium 199
description With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture	description HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture	description HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture	description 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form powder	form liquid	form powder	form liquid
components L-glutamine: yes phenol red: yes NaHCO₃: no sodium pyruvate: no Earle’s salts (5% CO₂): yes HEPES: no	components L-glutamine: no sodium pyruvate: no HEPES: 25 mM phenol red: yes NaHCO₃: yes Earle’s salts (5% CO₂): yes	components L-glutamine: yes phenol red: yes Earle’s salts (5% CO₂): yes NaHCO₃: no sodium pyruvate: no HEPES: 25 mM	components NaHCO₃: no phenol red: yes sodium pyruvate: no HEPES: no Earle’s salts (5% CO₂): yes L-glutamine: no
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
Quality Level 500	Quality Level 400	Quality Level 500	Quality Level 400

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Storage Class

13 - Non Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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