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Malic Dehydrogenase from porcine heart

Name: Malic Dehydrogenase from porcine heart
Brand: SIGMA

buffered aqueous glycerol solution, 600-1000 units/mg protein (biuret)

Synonym(s):

L-Malate: NAD⁺ oxidoreductase, MDH, Malate Dehydrogenase

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

1.1.1.37 (BRENDA, IUBMB)

MDL number:

MFCD00131567

Specific activity:

600-1000 units/mg protein (biuret)

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Properties

form

buffered aqueous glycerol solution

Quality Segment

200

specific activity

600-1000 units/mg protein (biuret)

cation traces

NH₄⁺: ≤10 μg/mg protein

foreign activity

Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%

shipped in

wet ice

storage temp.

2-8°C

Description

General description

Malic dehydrogenase is a mitochondrial isozyme and an important catalyst in the tricarboxylic acid cycle.

Mitochondrial Isozyme

Application

Malic dehydrogenase has been used in a study to assess the comparative molluscicidal action of Ginko biloba extract on snail hosts. It has also been used in a study to investigate the effect of early feed restriction on metabolic programming and compensatory growth in broiler chickens. [1]

Biochem/physiol Actions

Eukaryotic cells contain two different isozymes of malate dehydrogenase: mitochondrial (m-MDH) and soluble or cytoplasmic (s-MDH). This product consists of the mitochondrial form.
The enzyme catalyzes the following reaction:Oxaloacetate + β-NADH → L-Malate + β-NAD

Malic Dehydrogenase can be reversibly inactivated by treatment with pyridoxal 5′-phosphate at 25 °C. [2]

Physical form

Solution in 50% glycerol containing 0.05 M potassium phosphate buffer, pH 7.5

Other Notes

One unit will convert 1.0 μmole of oxalacetate and β-NADH to _L-malate and β-NAD per min at pH 7.5 at 25 °C.

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This Item	M1567	M9004	M7383
Sigma-Aldrich M2634 Malic Dehydrogenase from porcine heart	Sigma-Aldrich M1567 Malic Dehydrogenase from porcine heart	Sigma-Aldrich M9004 Malic Dehydrogenase from bovine heart	Sigma-Aldrich M7383 Malic Dehydrogenase from porcine heart
specific activity 600-1000 units/mg protein (biuret)	specific activity ≥600 units/mg protein (biuret)	specific activity 2000-4000 units/mg protein (modified Warburg-Christian)	specific activity ≥400 units/mg protein (biuret)
form buffered aqueous glycerol solution	form ammonium sulfate suspension	form ammonium sulfate suspension	form ammonium sulfate suspension
shipped in wet ice	shipped in -	shipped in -	shipped in -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
foreign activity Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%	foreign activity Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%	foreign activity -	foreign activity Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%
cation traces NH₄⁺: ≤10 μg/mg protein	cation traces -	cation traces -	cation traces -

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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