When reconstituted and filter sterilized, this medium is expected to have a similar shelf-life as prepared 1X Medium 199 with Hanks salts and L-glutamine. See product 51322C for the prepared liquid medium. This product has an expiration date of 1 year from the date of quality release. Upon the addition of serum or other heat-sensitive components, the shelf life would be limited to 1 - 2 weeks at 2 - 8°C. Freeze/thaw cycles may result in precipitate formation.
M0393
Medium 199
With Hanks′ salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
M199 Medium, TCM 199
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Pricing and availability is not currently available.
Product Name
Medium 199, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Quality Segment
form
powder
technique(s)
cell culture | mammalian: suitable
components
HEPES: no
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
NaHCO3: no
L-glutamine: yes
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
General description
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Application
Medium 199 has been used:
- to test and determine the pH sensitivity of the sensors in a complex environment
- as a culture medium during oocyte harvesting
- as a component of Leishmania donovani (LdBOB) promastigote media for culturing L. donovani
- to culture visceral adipose tissue explants
Preparation Note
Formulated to contain 10.6 grams of powder per liter of medium.
Supplement with 0.35 g/L sodium bicarbonate.
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This Item | |||
|---|---|---|---|
| form powder | form powder | form powder | form liquid |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| components HEPES: no | components L-glutamine: yes | components L-glutamine: no | components sodium pyruvate: no |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 500 | Quality Level 500 | Quality Level 500 | Quality Level 400 |
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Once prepared, how long can the media stay refrigerated? Can it be frozen?
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