Since you plan to use an amount between 15-30 mg, the simplest method is to weigh the lipase resin using an analytical balance. Ensure the balance is calibrated and zeroed before weighing. Use a small container or weighing paper to avoid direct contact with the resin.
L4777
Lipase acrylic resin
≥5,000 U/g, recombinant, expressed in Aspergillus niger
Synonym(s):
Novozyme 435
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-619-9
MDL number:
Specific activity:
≥5,000 U/g
Recombinant:
expressed in Aspergillus niger
Pricing and availability is not currently available.
recombinant
expressed in Aspergillus niger
Quality Segment
form
solid
specific activity
≥5,000 U/g
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
matrix
macroporous acrylic resin
application(s)
diagnostic assay manufacturing
greener alternative category
storage temp.
2-8°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
General description
Lipases belong to the class of hydrolases. Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.
Lipase from produced by submerged fermentation of a genetically modified Aspergillus niger microorganism and adsorbed on a macroporous resin.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.
Application
Lipase acrylic resin has been used:
- as a catalyst to carry out the esterification of pLACT with butyric acid
- as an additive in fully hydrogenated canola oil (FHCO) and high oleic sunflower oil (HOSO) blend for enzymatic interesterification
- to study its effect on the degree of oleic acid conversion upon esterification
Immobilized preparation of a thermostable lipase, particularly useful in the synthesis of esters and amides, and has a broad substrate specificity.
Biochem/physiol Actions
Lipases are used in detergent, food, leather, pharmaceutical and dairy industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis. Candida antarctica lipase B is considered as an important enzyme due to its use in esterification, hydrolysis, aminolysis and transesterification.
Other Notes
The material may be reused for identical reactions. It is not recommended for use in different reactions due to the probability of cross-contamination and loss of activity. There is no estimation as to the number of times the product may be used.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥5,000 U/g | specific activity ≥40,000 units/mg protein | specific activity ≥2 U/mg | specific activity ≥700 unit/mg solid |
| form solid | form lyophilized powder | form powder | form lyophilized |
| recombinant expressed in Aspergillus niger | recombinant - | recombinant - | recombinant - |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) sample preservation | application(s) - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
If using the enzyme in an academic organic chemistry teaching lab, what is the best method of measurement of the enzyme quantity? Planned amount is 15-30 mg.
1 answer-
Helpful?
-
-
Hello. I would like to ask if this lipase is Lipase B from Candida Antarctica, as this is not specified in the SDS or the specification sheet.
1 answer-
This product is produced by submerged fermentation of a genetically modified Aspergillus niger microorganism and adsorbed on a macroporous resin.
Helpful?
-
-
Is the supported enzyme reusable, and if so in which conditions?
1 answer-
This product is supplied by Novozymes and is commercially available as Novozym 435. The material may be reused for identical reactions. It is not recommended for use in different reactions due to the probability of cross-contamination and loss of activity. There is no estimation as to the number of times the product may be used. The link below may be useful in regeneration. This information has not been validated.
https://www.researchgate.net/figure/Regeneration-of-deactivated-Novozyme-435_fig7_313863272
Helpful?
-
-
What is the substrate specificity of Product L4777, Lipase acrylic resin?
1 answer-
Lipase acrylic resin has a broad substrate specificity. It promotes reactions between a wide range of primary and secondary alcohols and carboxylic acids.
Helpful?
-
-
What solvents are most compatible with Product L4777, Lipase acrylic resin?
1 answer-
This resin works well in solvent-free reactions, as well as in inert water immiscible solvents such as petroleum ether, n-hexane and n-heptane. If the reaction being catalyzed is hydrolysis of an ester, addition of water to the reaction mix is recommended. For other reactions water is likely not required, and in some cases would actually be detrimental.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
What are the components of Product L4777, Lipase acrylic resin, and what are the optimal recommended conditions?
1 answer-
This product consists of a thermostable lipase enzyme immobilized on a macroporous acrylic bead resin. The bead-shaped particles have a diameter in the range of 0.3-0.9 mm. The resin will be compatible with stearyl alcohol at temperatures of 40-60°C, which is the normal operating temperature of the enzyme. This immobilized enzyme can be used in hexane with no adverse effects.
Helpful?
-