I2002
Isocitric Dehydrogenase (NADP) from porcine heart
Type IV, buffered aqueous glycerol solution, 3-20 units/mg protein
Synonym(s):
threo-Ds-Isocitrate: NADP+ oxidoreductase, ICDH, IDH
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
200-289-5
MDL number:
Specific activity:
3-20 units/mg protein
type
Type IV
form
buffered aqueous glycerol solution
quality
≤ 0.2% of main activity is obtained with NAD as cofactor.
specific activity
3-20 units/mg protein
composition
Protein biuret
shipped in
wet ice
storage temp.
2-8°C
Application
Isocitric dehydrogenase (IDH) has been used:
- for in vitro activity assay to study the effects of thorium on citric acid cycle enzymes in the mitochondrial respiration pathway
- as a standard to measure the impact of temperature on nicotinamide diphosphate (NADP)-IDH activity
- in isocitrate assay
Biochem/physiol Actions
Isocitrate dehydrogenase (IDH) is a homodimeric enzyme and participates in several processes such as adapting to hypoxia, histone demethylation, and DNA modification. This enzyme has three isoforms like IDH1, IDH2, and IDH3.
Physical form
Solution in 50% glycerol in EDTA buffer salts, pH 6.0
Other Notes
One unit will convert 1.0 μmole of isocitrate to α-ketoglutarate per min at pH 7.4 at 37 °C.
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Storage Class
10 - Combustible liquids
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Icksoo Lee et al.
Biochimica et biophysica acta, 1802(2), 275-283 (2009-10-20)
Noonan syndrome (NS) is an autosomal dominant disorder, and a main feature is congenital heart malformation. About 50% of cases are caused by gain-of-function mutations in the tyrosine phosphatase SHP2/PTPN11, a downstream regulator of ERK/MAPK. Recently it was reported that
Avtar Singh et al.
The Journal of biological chemistry, 278(35), 33208-33216 (2003-06-07)
Transhydrogenase couples the reduction of NADP+ by NADH to inward proton translocation across mitochondrial and bacterial membranes. The coupling reactions occur within the protein by long distance conformational changes. In intact transhydrogenase and in complexes formed from the isolated, nucleotide-binding
Sergiy M Nadtochiy et al.
The Journal of biological chemistry, 291(38), 20188-20197 (2016-08-12)
2-Hydroxyglutarate (2-HG) is an important epigenetic regulator, with potential roles in cancer and stem cell biology. The d-(R)-enantiomer (d-2-HG) is an oncometabolite generated from α-ketoglutarate (α-KG) by mutant isocitrate dehydrogenase, whereas l-(S)-2-HG is generated by lactate dehydrogenase and malate dehydrogenase
Global Trade Item Number
| SKU | GTIN |
|---|---|
| I2002-25MG | 04061832730448 |
| I2002-250MG | 04061832730431 |
| I2002-5G-A | 04061832490205 |
| I2002-25G-A | 04061833848241 |
| I2002-100MG | 04061833848234 |
| I2002-500MG | 04061832730455 |