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G4166

Glucose Isomerase from Streptomyces murinus

≥350 U/g

Synonym(s):

Sweetzyme® IT Extra, D-xylose ketol-isomerase

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
≥350 U/g
Biological source:
Streptomyces sp. (S. murinus)
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biological source

Streptomyces sp. (S. murinus)

Quality Segment

form

powder

specific activity

≥350 U/g

storage temp.

2-8°C

General description

0.33 g yields an approximate bed volume of 1ml
Immobilized glucose isomerase produced from a selected strain of Streptomyces murinus.

Application

Immobilized glucose isomerase produced from Streptomyces murinus was used for the isomerization of xylose.[1] Glucose isomerase is used in the food industry to produce high-fructose corn syrup[2].
Glucose Isomerase from Streptomyces murinus has been used to isomerize xylose to xylulose during the production of dihydrogen from Xylose. It has also been used in the synthetic enzymatic pathway for dihydrogen production from sucrose, to catalyze isomerization of glucose to fructose.

Biochem/physiol Actions

Few anaerobic bacteria, fungi and plants express an intracellular metalloenzyme called D-xylose isomerase (XI). Most bacteria use the enzyme D-xylose isomerase to transform D-xylose to D-xylulose. D-Xylose isomerase (XI) converts the aldo-sugars xylose and glucose to their keto analogs xylulose and fructose.[3]
Glucose isomerase has wide variety of industrial applications such as producing high-fructose corn syrup (HFCS) and production of ethanol from hemicelluloses. In addition, it also facilitates the study of structure-function relationships by advanced biochemical and genetic engineering techniques.

Other Notes

one unit converts glucose to fructose at an initial rate of 1 μmole per min at standard analytical conditions

Legal Information

A product of Novozyme Corp.
Sweetzyme is a registered trademark of Novozymes Corp.

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P5381P9544T2507
biological source

Streptomyces sp. (S. murinus)

biological source

bakers yeast

biological source

rabbit muscle

biological source

-

specific activity

≥350 U/g

specific activity

≥400 units/mg protein (biuret)

specific activity

≥200 units/mg protein

specific activity

~10,000 units/mg protein

form

powder

form

ammonium sulfate suspension

form

lyophilized powder

form

ammonium sulfate suspension

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

1–2 of 2 Questions  
  1. Is G4166 an immobilized enzyme? If it is, what support is it immobilized on?

    1 answer
    1. This is a Novozyme product, Sweetzyme® IT Extra, which is an immobilized enzyme. It is glutaraldehyde crosslinked on polyethyleneimine, and intended to be used in a continuous fixed bed process (converting high dextrose syrup to high fructose syrup).

      Helpful?

  2. グルコースイソメラーゼ Streptomyces murinus由来(Cat.No G4166) が可溶化できません。どのように溶かすのでしょうか?またどのような溶液だと可能でしょうか?

    1 answer
    1. This is a product of Novozymes, available commercially as Sweetzyme. This is an immobilized enzyme used in a fixed bed process to convert dextrose syrup to fructose syrup. This material is in granular form with a particle size range of 0.3-1.0 mm. This material is not intended to be solubilized.

      Helpful?

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