Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
E5134
Ethylenediaminetetraacetic acid disodium salt dihydrate
suitable for electrophoresis, for molecular biology, 99.0-101.0% (titration)
Synonym(s):
Sequestrene Na₂, Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2
About This Item
Recommended Products
grade
for molecular biology
Quality Level
Assay
99.0-101.0% (titration)
form
powder
reaction suitability
reagent type: chelator
technique(s)
electrophoresis: suitable
impurities
≤0.005% Insolubles
≤0.1% Nitrilotriacetic acid (NTA)
mp
248 °C (dec.) (lit.)
solubility
3 M NaOH: 1.6 g/10 mL
cation traces
Fe: ≤0.005%
Pb: ≤0.002%
suitability
suitable for electrophoresis
suitable for molecular biology
foreign activity
DNase, RNase, NICKase and protease, none detected
storage temp.
room temp
SMILES string
O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
InChI
1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2
InChI key
OVBJJZOQPCKUOR-UHFFFAOYSA-L
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General description
Application
- as a component in buffer solutions for electrophoresis, cell culture, enzymatic assays, siRNA, flow cytometry, dimetylmethylene blue, and more[2]
- an anticoagulant for whole blood collection[1]
- a stabilizer solution for freezing cell culture samples[3]
- in combination with PBS and trypsin, to trypsinize cells[4]
- for phytoextraction of heavy metals in polluted soils[5]
Features and Benefits
- Molecular Biology grade, suitable for many molecular biology applications
- Free of endonucleases DNase, RNase, Nickase, and Protease
Other Notes
comparable product
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - STOT RE 2 Inhalation
Target Organs
Respiratory Tract
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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Certificates of Analysis (COA)
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How is shipping temperature determined? And how is it related to the product storage temperature?
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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Product E5134 has a re-test date of Aug. 2023. Has the re-test been completed?
1 answer-
The specific lot number is required to determine the retest status. You may obtain this lot status by contacting our Technical Service team. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquires" under the Products Section with all the required information, including the specific lot number, so that a member of our team can reach out to you to assist further. Thank you.
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Is Product E5134, EDTA disodium salt, stable in solution?
1 answer-
Yes. A 0.5 M stock solution at pH 8.5 is stable for months at 2-8°C.
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Can solutions of Product E5134, EDTA disodium salt, be autoclaved?
1 answer-
Yes.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the working concentration for Product E5134, EDTA disodium salt, for use as an anti-coagulant?
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The optimal concentration is 1.5 mg per mL of blood.
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