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E0760

Sigma-Aldrich

Endothelial cell growth supplement from bovine pituitary

Bioreagent, powder, suitable for cell culture

Synonym(s):

ECGS, Endothelial Mitogen

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

product name

Endothelial cell growth supplement from bovine pituitary, ECGS, suitable for cell culture, BioReagent

biological source

bovine pituitary glands

Quality Level

200

product line

BioReagent

form

powder

potency

2-300 μg/mL ED50/EC50

quality

endotoxin tested

packaging

pkg of 15 mg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤20 EU/vial (LAL test)

color

brown to tan

solubility

water: soluble to hazy, red to red-brown (1 vial reconstituted with 1 ml 0.85% NaCl)

storage temp.

2-8°C

General description

Endothelial cell growth supplement (ECGS) is an extract of bovine pituitary glands containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of a published method. It is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.

Application

Endothelial cell growth supplement from bovine pituitary has been used:
  • as a component in Roswell park memorial institute (RPMI) 1640 medium for endothelial cell culture
  • as a supplement in medium 199 for endothelial cell culture
  • as a supplement in Dulbecco′s modified eagle medium (DMEM):Ham′s F-12
  • as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) for human umbilical vein endothelial cells (HUVEC) culture

Biochem/physiol Actions

Endothelial cell growth supplement (ECGS) has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H361fd

Hazard Classifications

Repr. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Surface staining and cytotoxic activity of heat-shock protein 60 antibody in stressed aortic endothelial cells.
Xu Q, et al.
Circulation Research, 75(6), 1078-1085 (1994)
In vitro biosynthesis of leukemia inhibitory factor/human interleukin for DA cells by human endothelial cells: differential regulation by interleukin-1 alpha and glucocorticoids
Grosset C, et al.
Blood, 86(10), 3763-3770 (1995)
Autoantibodies against heat shock protein 60 mediate endothelial cytotoxicity.
Schett G, et al.
The Journal of Clinical Investigation, 96(6), 2569-2577 (1995)
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Pathological vascular endothelial damage caused by hypoxia is the basis of many vascular-related diseases. However, the role of circular RNA in hypoxic vascular injury is still poorly understood. Here, we found that hypoxia induced AFF1 circular RNA (circAFF1) can activate
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