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D5751

Blue Dextran

Void volume marker for gel filtration columns.

Synonym(s):

Dextran blue from Leuconostoc mesenteroides

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About This Item

CAS Number:
UNSPSC Code:
12352200
NACRES:
NA.25
MDL number:
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biological source

non-animal source

Quality Level

form

powder

quality

200

mol wt

2,000,000

technique(s)

affinity chromatography: suitable

suitability

suitable for GFC marker

General description

Blue Dextran is a soluble derivative of dextran. Its structure includes triazinyl dye Cibacron blue F3GA covalently bound to high molecular weight (2000 kDa) dextran. Blue Dextran is generally used as a marker to determine void volume in gel filtration chromatography columns.

Application

Blue Dextran has been used for calibration of size exclusion chromatography (SEC) column. It has also been used in Dextran blue permeability assays to determine the integrity of co-cultures containing epithelial cells. Blue dextran may be used in affinity chromatography, gel filtration chromatography, protein chromatography, and molecular weight markers. Blue dextran is also suitable to study Alzheimer′s disease.

Preparation Note

Prepared from dextran with an average mol. wt. of 2,000,000.

Analysis Note

degree of substitution: 0.10 to 0.12 mmol Cibracon Blue per gram of dextran

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This Item
D47729000803714
biological source

non-animal source

biological source

-

biological source

synthetic

biological source

synthetic

technique(s)

affinity chromatography: suitable

technique(s)

-

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

form

powder

form

powder

form

powder

form

powder

suitability

suitable for GFC marker

suitability

-

suitability

-

suitability

-

mol wt

2,000,000

mol wt

~2,000,000

mol wt

average mol wt 5,000

mol wt

average mol wt 20,000


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Renata G F Alvim et al.
Biotechnology and bioengineering, 118(9), 3581-3592 (2021-06-19)
Yellow fever (YF) is a life-threatening viral disease endemic in parts of Africa and Latin America. Although there is a very efficacious vaccine since the 1930s, YF still causes 29,000-60,000 annual deaths. During recent YF outbreaks there were issues of
Wenjun Zhang et al.
Analytica chimica acta, 1191, 339358-339358 (2022-01-17)
Size exclusion chromatography (SEC) is one of the most commonly used techniques to detect the molecular weight (MW) of dissolved organic matter (DOM) in aquatic environments. The significant improvement and focus of this method have been the application of multiple
Elementary steps in synaptic transmission revealed by currents through single ion channels.
B Sakmann
Bioscience reports, 12(4), 237-262 (1992-08-01)



Global Trade Item Number

SKUGTIN
D5751-5G04061833586754
D5751-1G04061833586747
D5751-10G04061833586730

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