EMT6/MTXR
96042322, mouse unknown/unspecified, Epithelial-like
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About This Item
UNSPSC Code:
41106514
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product name
EMT6/MTXR, 96042322
biological source
mouse unknown/unspecified
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse mammary, drug-resistant
Cell Line Description
The drug-resistant cell line EMT6/MTXR was derived from the mouse mammary tumour cell line EMT6/P (Sigma Catalogue number. 96042344) by growth in increasing concentrations of methotrexate. EMT6/MTXR did not hyperexpress P-glycoprotein and did not show cross-resistance to other drugs of the multi-drug resistance (MDR) phenotype. It is recommended to culture the cells without drug on resuscitation until first passage or growth has been fully established.
Application
Drug-resistance studies
Culture Medium
MEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1μg/ml methotrexate + 15% Foetal Bovine Serum (FBS).. Resuscitate in drug-free medium; add drug once growth is fully established
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA;5% CO2; 37ºC
Other Notes
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Certificates of Analysis (COA)
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