C7082

Monoclonal Anti-β-Catenin antibody produced in mouse

clone 6F9, ascites fluid

• Synonym(s): Anti-CTNNB, Anti-EVR7, Anti-MRD19, Anti-NEDSDV, Anti-armadillo
• MDL number: MFCD00282178
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 6F9, monoclonal
• mol wt: antigen 94 kDa
• contains: 15 mM sodium azide
• species reactivity: human, chicken, canine, bovine
• technique(s): indirect immunofluorescence: 1:1,000 using cultured MDBK cells; microarray: suitable; western blot: 1:1,000 using cultured MDBK cells extract
• isotype: IgG1
• UniProt accession no.: P35222
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CTNNB1(1499)

Description

General description

Monoclonal Anti- β -Catenin (mouse IgG1 isotype) is derived from the 6F9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. CTNNB1 (catenin β 1) gene is mapped to human chromosome 3p21. The protein encoded is also called as cadherin-associated protein.The encoded protein β-catenin is localized to the nucleus. Monoclonal antibody reacting specifically with β -catenin is an essential tool in defining the interactions and distributions of β -catenin and its relationships with other catenins and cadherins in various cells and tissues.

Specificity

The antibody does not cross-react with plakoglobin (γ-catenin).

Immunogen

recombinant chicken β-catenin.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Monoclonal Anti-β-Catenin antibody produced in mouse has been used in:

• immunohistochemistry
• immunofluorescence
• immunoblotting

Biochem/physiol Actions

In Wnt/β-catenin signal pathway, β-Catenin participates as a signal transduction molecule. The pathway is associated with development, tissue homeostasis and oncogenic transcription. Upregulation of the gene is associated with glioma cell proliferation. The encoded protein has dual function that regulates the coordination of cell-cell adhesion by linking cytoskeleton and cellular junctions and gene transcription. Wnt/β-catenin signal pathway is known to induce progression of colon cancer progression. It is useful for differential diagnosis of Osteoblastoma (OB) and osteosarcoma (OS). β-catenin signaling pathway may contribute to cholangiocarcinoma (CCA) cell proliferation. Monoclonal antibody reacting specifically with β -catenin is an essential tool in defining the interactions and distributions of β -catenin and its relationships with other catenins and cadherins in various cells and tissues.

Physical form

Supplied as ascites fluid with 15 mM sodium azide as a preservative.

Storage and Stability

For extended storage freeze in working aliquots. For continuous use, store at 2-8 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves