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C6357

Sigma-Aldrich

Caspase 4 human

recombinant, expressed in E. coli, 5,000 units/mg protein

Synonym(s):

ICE(rel)II, Ich-2, TX

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About This Item

Enzyme Commission number:
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.32

recombinant

expressed in E. coli

form

solution

specific activity

5,000 units/mg protein

mol wt

30 kDa

solubility

PBS: soluble

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CASP4(837)

General description

The caspase 4 (CASP4) gene is mapped to human chromosome 11q21. Caspase 4 is a cysteine-aspartic protease, localized to the outer membrane of the endoplasmic reticulum (ER). The N-terminus of the enzyme contains a caspase recruitment domain (CARD).

Biochem/physiol Actions

Caspase 4 is an inflammatory caspase associated with innate immune responses. It plays a key role in the endoplasmic reticulum (ER) stress-induced apoptosis. Caspase 4 mediates the fusion of phagosomes containing pathogens with the lysosome. It inhibits pathogen replication, development, and production of pro-inflammatory cytokines. Caspase 4 induces caspase activation and pyropoptotic death by binding to the intracellular lipopolysaccharide (LPS) of the Gram-negative bacterial outer membrane. This is mediated by the highly specific caspase recruitment domain (CARD).
Useful in screening caspase inhibitors, studying enzyme kinetics and regulation, determining target substrates, as well as serving as positive controls in caspase activity assays and Western blot analysis.

Unit Definition

One unit will hydrolyze 1 nmol of the caspase substrate WEHD-pNA to WEHD and p-nitroaniline per hour at pH 7.2 at 37 °C.

Physical form

Solution in 0.052% ammonium chloride, 0.158% Tris−HCl, and 0.76% sodium chloride

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Giuliana Papoff et al.
Scientific reports, 8(1), 17705-17705 (2018-12-12)
Inflammatory caspases, including human caspase-4 (CASP4), play key roles in innate immune responses to promote fusion of phagosomes harboring pathogenic bacteria with lysosomes, halt intracellular replication of pathogens, maturation and secretion of pro-inflammatory cytokines. The role of inflammatory caspases in
Itziar Salaverria et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 25(10), 1216-1222 (2007-02-14)
To compare the genetic relationship between cyclin D1-positive and cyclin D1-negative mantle cell lymphomas (MCLs) and to determine whether specific genetic alterations may add prognostic information to survival prediction based on the proliferation signature of MCLs. Seventy-one cyclin D1-positive and
Caspase-4 and Caspase-5
Handbook of Proteolytic Enzymes, 2265-2269 (2013)

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