B5388

Brilliant Cresyl Blue ALD

Dye content, ≥60%, certified by the Biological Stain Commission, powder

• Synonym(s): 3-Amino-7-(diethylamino)-2-methylphenoxazin-5-ium (T-4)-tetrachlorozincate (2:1), Brilliant Blue C, Brilliant Cresyl blue
• Linear Formula: C₁₇H₂₀N₃OCl · 1/2ZnCl₂
• CAS Number: 51716-96-2
• Molecular Weight: 385.96
• NACRES: NA.47
• PubChem Substance ID: 24891836
• UNSPSC Code: 12171500
• EC Number: 279-675-0
• MDL number: MFCD00148901
• Beilstein/REAXYS Number: 5690713
• Colour Index Number: 51010

Properties

• Product Name: Brilliant Cresyl Blue ALD, certified by the BSC
• agency: certified by the BSC
• Quality Segment: 200
• form: powder
• composition: Dye content, ≥60%
• solubility: water: 1 mg/mL, blue to very deep blue
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Zn-2](Cl)(Cl)(Cl)Cl.N(CC)(CC)c4cc5[o+]c6c(nc5cc4)cc(c(c6)N)C.N(CC)(CC)c1cc2[o+]c3c(nc2cc1)cc(c(c3)N)C
• InChI: 1S/2C17H20N3O.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;/h2*6-10H,4-5,18H2,1-3H3;4*1H;/q2*+1;;;;;+2/p-4
• InChI key: SIHASHFMDFXGSX-UHFFFAOYSA-J

Description

Application

Certified as a supravital stain for reticulocytes.

Brilliant Cresyl Blue ALD has been used for the selection of oocytes. It has also been used for the selection of cumulus-oocyte complexes (COCs).

Biochem/physiol Actions

Brilliant Cresyl Blue ALD is mainly used for the selection of oocytes. It helps in determining the activity of glucose-6-phosphate dehydrogenase (G6PD). This enzyme is generated in growing oocytes. However, oocytes which complete their growth have low levels of G6PD. Low G6PD activity results in blue coloration of the cytoplasm because brilliant cresyl blue is not reduced to a colourless compound. It is also used for the staining in reticulocytes, where the dye stains reticulin.

Other Notes

The structure of Brilliant Cresyl Blue ALD contains a 2-methyl modification on the phenoxazine core, while the structure of the historic Brilliant Cresyl Blue contains a 8-methyl modification on the phenoxazine core. The biological staining application is the same for both structures.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves