This enzyme is soluble in aqueous buffers and water, at 20 mg/mL. This material may also be prepared 100 mM Tris HCl buffer, pH 8.0, containing 0.02% BSA (0.3 mg/ml), yielding a clear, colorless solution.
A9770
Aldehyde Dehydrogenase, potassium-activated from yeast
lyophilized powder, ≥10 units/mg protein
Synonym(s):
ALDH, CoA-independent aldehyde dehydrogenase, m-methylbenzaldehyde dehydrogenase, Aldehyde:NAD[P]+ oxidoreductase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-846-3
MDL number:
EC Number:
Specific activity:
≥10 units/mg protein
Biological source:
yeast
Pricing and availability is not currently available.
biological source
yeast
Quality Segment
form
lyophilized powder
specific activity
≥10 units/mg protein
storage condition
(Tightly closed. Dry)
color
white
UniProt accession no.
foreign activity
NADH oxidase ≤0.01%, NADPH oxidase ≤0.01%, alcohol dehydrogenase ≤0.01%, lactic dehydrogenase ≤0.01%
storage temp.
2-8°C
General description
Aldehyde dehydrogenase, from yeast, is used to catalyze the oxidation of a wide range of substrates, such as acetaldehyde, formaldehyde, propionaldehyde, n-butylaldehyde, isobutylaldehyde, n-valeraldehyde, caproaldehyde, benzaldehyde, glycoaldehyde, D-glyceraldehyde, malonic semialdehyde, and succinic aldehyde. Aldehyde dehydrogenase, from yeast has been used to study the production of ethanol and isobutanol. Aldehyde dehydrogenase, from Sigma, has been used along with alcohol dehydrogenase to measure ethanol production during the characterization of glycolytic metabolism and ion transport in Candida albicans.
The family of aldehyde dehydrogenase (ALDH) contains 19 genes in humans. It is localized in the nucleus, cytosol, mitochondria and endoplasmic reticulum of the cell.
Research area: Cell Signaling
Research area: Cell Signaling
Application
Biochem/physiol Actions
Aldehyde dehydrogenase (ALDH) modulates the non-P450 aldehyde reduction enzyme system. It protects the cell from the effects of toxic aldehydes. Mutations in this gene leads to Sjogren‐Larsson syndrome, Larsson syndrome, type II hyperprolinemia and cancer. ALDH-2 lowers cardiac ischemia, which arises due to myocardial infarction or post cardiac surgery.Aldehyde Dehydrogenase (ALDH) participates in the functioning of regulatory T cells that are a part of the immune system. It is also involved in cellular detoxification, the amino acid metabolism and protects cells against ultraviolet (UV) rays-induced damage. ALDH plays a role in retinoic acid biosynthesis and signaling.
Aldehyde dehydrogenase is a tetramer and has several different isoforms. Aldehyde dehydrogenase is inhibited by propylurea, crotonaldehyde, n-propyl isocyanate, cyclohexyl isocyanate, 1-n-propyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea, and 1-methyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea. The enzyme tested in 0.01 M pyrophosphate buffer shows a sharp optimum around pH 9.3 with acetaldehyde as substrate.
Physical form
Contains potassium phosphate salts
Other Notes
One unit will oxidize 1 micromole of acetaldehyde to acetic acid per minute pH 8.0 at 25 deg C.
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This Item | |||
|---|---|---|---|
| description lyophilized powder, ≥10 units/mg protein | description from yeast | description lyophilized powder, ≥2.0 units/mg protein | description ≥300 units/mg protein, lyophilized powder (contains buffer salts), Mw 141-151 kDa |
| biological source yeast | biological source yeast | biological source - | biological source Saccharomyces cerevisiae |
| specific activity ≥10 units/mg protein | specific activity ~20 units/mg protein (At 25 °C with acetaldehyde as the substrate.) | specific activity ≥2.0 units/mg protein | specific activity ≥300 units/mg protein |
| form lyophilized powder | form lyophilized | form lyophilized powder | form lyophilized powder (contains buffer salts) |
| storage condition (Tightly closed. Dry) | storage condition - | storage condition - | storage condition (Keep container tightly closed in a dry and well-ventilated place.) |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| UniProt accession no. | UniProt accession no. - | UniProt accession no. - | UniProt accession no. |
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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how to dissolve this enzyme and which solvent to use. Please share the protocol
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What is the molecular weight of Aldehyde Dehydrogenase, potassium-activated from baker's yeast (S. cerevisiae), product A9770?
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Aldehyde dehydrogenase from bakers yeast, product A9770, is a tetramer consisting of subunits of 57 kDa. The molecular weights of its monomer, dimer, trimer, and tetramer are 57 kDa, 114 kDa, 171 kDa, and 228 kDa respectively. The monomers are thought to be assembled into tetramers in a heterologous square arrangement.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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