A5795

Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): HRP Rabbit Anti-Rat IgG (whole molecule)
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162813
• Conjugate: peroxidase conjugate
• Clone: polyclonal
• Application: ELISA (d), IHC (p), WB CL
• Citations: 72

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: peroxidase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: rat
• should not react with: human
• technique(s): direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:750, western blot (chemiluminescent): 1:80,000-1:160,000 using rat PC12 cell extract
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.

IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Anti-Rat IgG (whole molecule)-Peroxidase antibody binds all rat Igs and does not react with human IgG.

Immunogen

purified rat IgG

Application

Anti-Rat IgG (whole molecule)-Peroxidase antibody has been used as a secondary antibody in immunohistochemical applications at a dilution of 1:1500. The product has also been used for western blot analysis at 1:20,000[1]. Furthermore, the antibody can be used for direct ELISA at 1:50,000.

Anti-Rat IgG (whole molecule)?Peroxidase antibody produced in rabbit has been used in immunohistochemistry and western blot analysis.[1]

HA-tagged proteins over expressed in S. cerevisiae were detected by western blot using HRP-conjugated rabbit anti-rat IgG as the secondary antibody.

Biochem/physiol Actions

Immunoglobulin (IgG) helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. IgG participates in hypersensitivity type II and type III.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Preparation Note

Adsorbed with human IgG to reduce background staining with human samples.

Prepared by two-step glutaraldehyde method.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable