This product is prepared by reaction of the diazotized form of Sulfanilamide with casein. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/768/303/a2765pis.pdf
A2765
Azocasein
protease substrate, chromogenic, powder
Synonym(s):
Sulfanilamide-azocasein
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About This Item
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Product Name
Azocasein, protease substrate
biological source
bovine
Quality Segment
form
powder
technique(s)
ligand binding assay: suitable
solubility
water: 5 mg/mL, clear, orange to very deep orange
ε (extinction coefficient)
≥25 at 440 nm in 0.1 M NaOH at 1%
storage temp.
2-8°C
General description
Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.
Application
Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.
Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.
Azocasein has been used as a substrate for determination of protease activity.
Other Notes
View more information on azocasein protease assay at www.sigma-aldrich.com/enzymeexplorer
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This Item | |||
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| solubility water: 5 mg/mL, clear, orange to very deep orange | solubility water: 10 mg/mL, clear to slightly hazy | solubility water: 5 mg/mL, clear to hazy, yellow to orange | solubility methanol: 20 mg/mL, clear, colorless to light yellow |
| technique(s) ligand binding assay: suitable | technique(s) - | technique(s) - | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form powder | form powder | form essentially salt-free, lyophilized powder | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| biological source bovine | biological source - | biological source - | biological source - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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A2765 Sigma-Aldrich アゾカゼイン この製品に含まれるアゾ色素を標品として使用したいのですが、どの化合物になるでしょうか？
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