The pack size indicates the number of units per vial. The volume of each package varies from lot to lot. The minimum concentration of this solution is 30 mg protein per milliliter. The activity may range from 10 – 40 units per milligram protein. This information is reported in the lot-specific Certificate of Analysis. At the minimum protein concentration and activity provided above, the volume of the 250UN package would be 0.833mL and the 1000UN package would contain 3.33mL. Higher activity and protein concentrations would result in lower volumes. Please see the link below to review a sample or lot-specific Certificate of Analysis:
https://www.sigmaaldrich.com/product/sigma/a2404#product-documentation
A2404
Alcohol Oxidase solution from Pichia pastoris
buffered aqueous solution, 10-40 units/mg protein (biuret)
Synonym(s):
Alcohol:oxygen oxidoreductase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
10-40 units/mg protein (biuret)
Pricing and availability is not currently available.
form
buffered aqueous solution
Quality Segment
specific activity
10-40 units/mg protein (biuret)
shipped in
dry ice
storage temp.
−20°C
General description
Alcohol oxidase (AOX) is a homo-octamer, which has a molecular weight of 600-kDa. It has eight flavin adenine dinucleotide (FAD) cofactors. It belongs to the glucose-methanol-choline (GMC) oxidoreductase family.
Application
Alcohol Oxidase solution from Pichia pastoris has been used in cotton pectin methylesterase (PME) enzyme assay. It has also been used to immobilize onto polyaniline (PANI) film as a visual biosensor in dip-stick format for the determination of ethanol in fermented beverage samples.
Alcohol Oxidase may be used to study protein translocation into peroxisomes. Product A2404 is from Pichia pastoris. It has been used for the bacterial expression and immunological verification of Hv-p68 cDNA clones [1].
Biochem/physiol Actions
Alcohol oxidase catalyzes the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes. It has the highest affinity for methanol. Alcohol oxidase is involved in the metabolism of methanol, which is important for yeasts that utilize methanol as their sole energy source. Alcohol oxidase is primarily found in the peroxisome but may be present in the cytoplasm as well.
Physical form
Solution in 30% sucrose with 0.1 M phosphate buffer at pH 8.0
Other Notes
One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C.
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This Item | |||
|---|---|---|---|
| specific activity 10-40 units/mg protein (biuret) | specific activity 5-15 units/mg protein | specific activity ≥30 units/mg protein (biuret) | specific activity ≥300 units/mg protein |
| form buffered aqueous solution | form lyophilized powder | form aqueous solution | form lyophilized powder (contains buffer salts) |
| shipped in dry ice | shipped in - | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −70°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Hello, Before ordering Alcohol Oxidase (Sigma A2404), could you confirm what “250 units” and “1000 units” mean? Do these indicate total units per vial, or a concentration (units per mL)? If total units, what is the typical fill volume per vial?
1 answer-
Helpful?
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What is the concentration of AOX in mg in 1 unit
1 answer-
The protein concentration in mg/mL and enzyme activity in unit/mg are lot-specific and reported on the product Certificate of Analysis.
Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/a2404#product-documentationHelpful?
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