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Sigma-Aldrich

Atto 647

BioReagent, suitable for fluorescence, ≥90% (HPCE)

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About This Item

MDL number:
MFCD05865408
UNSPSC Code:
12352108
NACRES:
NA.32

product line

BioReagent

Assay

≥90% (HPCE)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

transmittance

254 nm
647 nm

fluorescence

λex 643 nm; λem 665 nm in 0.1 M phosphate pH 7.0

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 645-651 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

General description

Atto 647 is a zwitterionic dye with a net electrical charge of zero. It is a pH sensitive product. While practically stable up to pH 8.5, it slowly degrades at higher pH. Atto 647 is a new label with high molecular absorption (120,000) and quantum yield (0.20) as well as enough Stokes shift between excitation and emission maximum. Atto 647 is characterized by a high photostability and brightness. In addition, this label has good water solubility and low triplet formation ability.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cytokinesis,Volume 137 of Methods in Cell Biology (2017)
Duncan Hill et al.
Biomedical microdevices, 13(4), 759-767 (2011-05-12)
This paper presents an overview of development of a novel disposable plastic biochip for multiplexed clinical diagnostic applications. The disposable biochip is manufactured using a low-cost, rapid turn- around injection moulding process and consists of nine parabolic elements on a
Daniel Puntener et al.
Journal of virology, 85(1), 481-496 (2010-11-05)
Human adenoviruses (Ads) replicate and assemble particles in the nucleus. They organize a linear double-strand DNA genome into a condensed core with about 180 nucleosomes, by the viral proteins VII (pVII), pX, and pV attaching the DNA to the capsid.
Jean-Manuel Segura et al.
The Journal of biological chemistry, 283(35), 24254-24263 (2008-06-27)
CD8(+) cytotoxic T lymphocytes (CTL) can recognize and kill target cells expressing only a few cognate major histocompatibility complex (MHC) I-peptide complexes. This high sensitivity requires efficient scanning of a vast number of highly diverse MHC I-peptide complexes by the
Emmanuel G Guignet et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 8(8), 1221-1227 (2007-04-17)
Sensitive live-cell fluorescence microscopy and single-molecule imaging are severely limited by rapid photobleaching of fluorescent probes. Herein, we show how to circumvent this problem using a novel, generic labeling strategy. Small nickel-nitrilotriacetate fluorescent probes are reversibly bound to oligohistidine sequences
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