56748

Imidazole

BioUltra, Molecular Biology, ≥99.5% (GC)

• Synonym(s): 1,3-Diaza-2,4-cyclopentadiene, Glyoxaline
• Linear Formula: C₃H₄N₂
• CAS Number: 288-32-4
• Molecular Weight: 68.08
• NACRES: NA.25
• PubChem Substance ID: 57651596
• eCl@ss: 39161001
• UNSPSC Code: 12352005
• EC Number: 206-019-2
• MDL number: MFCD00005183
• Beilstein/REAXYS Number: 103853

Properties

• grade: Molecular Biology
• Quality Segment: 200
• vapor pressure: <1 mmHg ( 20 °C)
• product line: BioUltra
• assay: ≥99.5% (GC)
• form: flakes, powder or crystals
• impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.2% water
• ign. residue (900 °C): ≤0.05%
• pH: 9.5-11.0 (25 °C, 0.1 M in H₂O)
• pKa (25 °C): 6.95
• bp: 256 °C (lit.)
• mp: 88-91 °C (lit.)
• solubility: H₂O: 0.1 M at 20 °C, clear, colorless
• anion traces: chloride (Cl^-): ≤50 mg/kg, sulfate (SO₄^2-): ≤50 mg/kg
• SMILES string: c1c[nH]cn1
• cation traces: Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg
• λ: 0.1 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.10, λ: 280 nm A_max: 0.10
• InChI: 1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)
• InChI key: RAXXELZNTBOGNW-UHFFFAOYSA-N

Description

Application

Excellent for buffers in the range of pH 6.2-7.8

Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for assay of horseradish peroxidase and as chelator for the binding of various divalent cations. Imidazole can be used for the elution of histidine containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for detection of proteins.

Other Notes

Review: As RNase model

Safety Information

• Pictograms: GHS08,GHS05,GHS07
• Signal Word: Danger
• Hazard Codes: H302,H314,H360D
• Precautionary Statements: P260 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 2
• Flash Point (°F): 293.0 °F - closed cup
• Flash Point (°C): 145 °C - closed cup
• PPE (personal protective equipment): Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges