21884

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate

BioReagent, suitable for fluorescence, ≥85% (HPCE)

• Synonym(s): 5&6DH-DA, CDFDA, Carboxy-DCFDA
• Empirical Formula (Hill Notation): C₂₅H₁₄Cl₂O₉
• CAS Number: 127770-45-0
• Molecular Weight: 529.28
• UNSPSC Code: 12352106
• NACRES: NA.32
• PubChem Substance ID: 57647949
• MDL number: MFCD08459585
• Beilstein/REAXYS Number: 7505106

Properties

• product line: BioReagent
• Quality Segment: 100
• assay: ≥85% (HPCE)
• solubility: DMF: soluble, DMSO: soluble, methanol: soluble
• fluorescence: λ_ex 470 nm; λ_em 529 nm in 0.1 M Tris pH 8.0 (esterase)
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5cc(ccc45)C(O)=O)c2cc1Cl.CC(=O)Oc6cc7Oc8cc(OC(C)=O)c(Cl)cc8C9(OC(=O)c%10ccc(cc9%10)C(O)=O)c7cc6Cl
• InChI: 1S/2C25H14Cl2O9/c1-10(28)33-21-8-19-15(6-17(21)26)25(14-4-3-12(23(30)31)5-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19;1-10(28)33-21-8-19-15(6-17(21)26)25(14-5-12(23(30)31)3-4-13(14)24(32)36-25)16-7-18(27)22(34-11(2)29)9-20(16)35-19/h2*3-9H,1-2H3,(H,30,31)
• InChI key: LZMPJBOMBCYYMP-UHFFFAOYSA-N

Description

General description

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate or CDFDA is a fluorescent oxidant sensing probe. It is an intracellular probe added to the cells as a pro-substrate, which upon hydrolysis by the esterase enzyme produces the fluorescent product called CDF.

Application

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate or Carboxy-DCFDA is used as a cell permeate probe to detect intracellular reactive oxygen species (ROS). The anionic fluorescent dye, 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate is suitable for studying Multi-Drug Resistance (MDR) by target cells.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Analysis Note

Exc_max: 504 nm (Lit.)

Other Notes

Intracellular esterase substrate with low sensitivity of fluorescence to intracellar pH

Safety Information

• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319,H335
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves