Skip to Content
Merck
All Photos(1)

Documents

MTOX1000CC24

Sigma-Aldrich

Caco-2 Engineered Control Cells

Human male colorectal tissue, adenocarcinoma

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106514

product name

Caco-2 Engineered Control Cells, one assay ready, 24-well plate

biological source

human male colorectal tissue (Source Disease: adenocarcinoma)

form

liquid

General description

The C2BBe1 cells, a sublcone of Caco-2 cells, correspond to ATCC CRL-2101. The C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years).

The three week production lead time begins on the Monday following a purchase, in the third week the plates are shipped on Tuesday for receipt on Wednesday or Thursday. As a biologic product that is shipped at room temperature the cells must be processed immediately upon receipt.

A minimum of 4 assay ready plates is required to purchase. Please allow for 1 week after ordering, for the plates to be seeded. Plates will be shipped to the customer on day 16 of the 21-day culturing process.

Application

The Engineered Control cells can be used for drug permeability assays. They may also be used as the control in conjunction with C2BBe1 Transporter Knockout cell lines for drug/transporter interaction studies. Engineered control cells have been used in the in vitro Caco-2 permeability assay. The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

Features and Benefits

The Caco-2 subclone, C2BBe1 Engineered Control cells, are ideal for permeability and transporter analyses as they express multiple transporters, are human derived and grow in a homogenous monolayer that forms tight junctions which is necessary for efflux ratio analysis. Other benefits include:
  • The 24-well Transwell format enables the these cells to be included in standard drug transporter protocols
  • Human assay with no interference from animal inhibitors
  • The 21-day cell culture period is reduced to 6-days for customers, saving valuable resources

Quality

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Legal Information

This product is covered under a consumable purchase agreement for one-time use only. For more information:ADME/Tox Cell Lines License

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

P Artursson
Journal of pharmaceutical sciences, 79(6), 476-482 (1990-06-01)
A human intestinal cell line, Caco-2, was used as a model to study the passive diffusion of drugs across intestinal epithelium. The cells formed continuous monolayers when grown on permeable filters of polycarbonate. After 10 days in culture, the monolayers
V Pade et al.
Journal of pharmaceutical sciences, 87(12), 1604-1607 (1999-04-03)
The objective of this investigation was to establish a relationship between drug permeability and solubility in vitro and the extent of drug absorption in humans. We selected drugs with varying permeabilities and solubilities with the aim of establishing a relationship
X Wu et al.
Pharmaceutical research, 17(2), 209-215 (2000-04-06)
The purpose of this study was to elucidate the mechanisms by which an HMG-CoA reductase inhibitor, atorvastatin (an organic acid with a pKa of 4.46), was transported in the secretory and absorptive directions across Caco-2 cell monolayers. Caco-2 cells were
S Yee
Pharmaceutical research, 14(6), 763-766 (1997-06-01)
To evaluate and optimize the use of Caco-2 cell monolayers to predict the in vivo absorption of a broad range of compounds in man. Caco-2 cells are derived from human adenocarcinoma colon cells and spontaneously differentiate when grown on porous
Kathleen E Sampson et al.
Drug metabolism and disposition: the biological fate of chemicals, 43(2), 199-207 (2014-11-13)
Membrane transporters P-glycoprotein [P-gp; multidrug resistance 1 (MDR1)], multidrug resistance-associated protein (MRP) 2, and breast cancer resistance protein (BCRP) affect drug absorption and disposition and can also mediate drug-drug interactions leading to safety/toxicity concerns in the clinic. Challenges arise with

Articles

We presents an article on The Role of Intestinal Efflux Transporters In Drug Absorption.

We presents an article on The Role of Intestinal Efflux Transporters In Drug Absorption.

We presents an article on The Role of Intestinal Efflux Transporters In Drug Absorption.

We presents an article on The Role of Intestinal Efflux Transporters In Drug Absorption.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service