Yes, DMSO is a suitable solvent for this material. Stock solutions are typically prepared at 10 mM, however concentrations of up to 20 mg/mL have been achieved.
Other solvents include Dimethylformamide, Chloroform, Acetonitrile, and Methanol. Stock solutions may be store in aliquots at -20 °C. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/401/542/d7008pis.pdf
D7008
Dihydroethidium
≥95% purity, powder
Synonym(s):
2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, Hydroethidine
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About This Item
Empirical Formula (Hill Notation):
C21H21N3
CAS Number:
Molecular Weight:
315.41
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
Beilstein/REAXYS Number:
5107482
Pricing and availability is not currently available.
Product Name
Dihydroethidium, ≥95%
Quality Segment
assay
≥95%
form
powder
technique(s)
titration: suitable
color
pink to very dark red
solubility
chloroform: 20 mg/mL
fluorescence
λex 370 nm; λem 420 nm (for cytoplasm of living cells), λex 535 nm; λem 610 nm (for chromatin of living cells)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
SMILES string
CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14
InChI
1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3
InChI key
XYJODUBPWNZLML-UHFFFAOYSA-N
Application
Dihydroethidium has been used as a superoxide probe in bovine pulmonary artery endothelial cell (BPAEC). It has been used to check the in situ generation of ROS (reactive oxygen species).
Redox indicator. Blue fluorescence until oxidized to ethidium.
Packaging
Packaged under Argon.
Other Notes
B-ring reduced analog of ethidium
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This Item | |||
|---|---|---|---|
| description ≥95% | description BioReagent, suitable for fluorescence, ≥95% (HPCE) | description fluorescent dye | description mitochondrial specific fluorescent dye |
| technique(s) titration: suitable | technique(s) - | technique(s) microbe id | staining: suitable | technique(s) - |
| color pink to very dark red | color - | color faint brown to brown, and Orange to Dark Orange and Red to Very Dark Red | color - |
| fluorescence λex 370 nm; λem 420 nm (for cytoplasm of living cells), λex 535 nm; λem 610 nm (for chromatin of living cells) | fluorescence λex 358 nm; λem 461 nm, λex 392 nm; λem 410 nm in methanol | fluorescence - | fluorescence - |
| solubility chloroform: 20 mg/mL | solubility methanol: soluble, acetonitrile: soluble | solubility H2O: 1 mg/mL | solubility ethanol: 1 mg/mL |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| form powder | form solid | form powder | form powder |
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Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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