Select histological procedures may specifically call for using Canada Balsam as the mounting media. Canada Balsam is sometimes applied "thickly", and the coverslip is omitted. If a coverslip is applied, fading might occur more quickly. For routine use, synthetic resinous mounting medias are most commonly used.
C1795
Canada balsam
Viscous Liquid
Synonym(s):
Balsam Canada
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About This Item
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Product Name
Canada balsam, Mounting medium for microscopy
Quality Level
form
viscous liquid
technique(s)
microbe id | staining: suitable
color
yellow to very dark yellow
refractive index
n20/D 1.522 (lit.)
density
0.99 g/mL
application(s)
hematology
histology
storage temp.
room temp
SMILES string
CC=CC=C(C=C)C(CN1CCC(NC(C1=O)CCCN=C(N)N)C(CC2=CC3=CC=CC=C3C=C2)NC(=O)C)C(=CC=C)C=CC
InChI
InChI=1S/C39H52N6O2/c1-6-10-16-30(9-4)34(32(14-7-2)15-8-3)27-45-24-22-35(44-36(38(45)47)19-13-23-42-39(40)41)37(43-28(5)46)26-29-20-21-31-17-11-12-18-33(31)25-29/h6-12,14-18,20-21,25,34-37,44H,2,4,13,19,22-24,26-27H2,1,3,5H3,(H,43,46)(H4,40,41,42)
InChI key
IIUZTTUQXFCORF-UHFFFAOYSA-N
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General description
Canada Balsam is a natural mounting medium obtained from balsam fir trees. It is produced from the resin of the balsam fir tree and its use can be combined with xylene-containing specimens. The optical properties of Canada Balsam are nearly identical to those of glass. Specimens must be dehydrated before using this medium. Permanent slides mounted with Canada Balsam have been stored for over a century.[1]
related product
Product No.
Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 2
Flash Point(F)
102.2 °F
Flash Point(C)
39 °C
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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For what applications is Canada Balsam, Product No. C1795, recommended?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Canada Balsam, Product No. C1795, a permanent mounting media or an aqueous mounting media?
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Canada Balsam is a permanent mounting media.
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Is Canada Balsam, Product No. C1795, commonly used in histology?
1 answer-
Canada Balsam is sourced from balsam fir trees (Abies balsamea). Costs are high for the naturally produced product. Synthetic mounting medias are cheaper in comparison. Since World War II, synthetic mounting medias have largely replaced naturally obtained products, such as Canada Balsam.
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How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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How does Canada Balsam, Product No. C1795, differ from a permanent mounting media - such as DPX Mountant for histology?
1 answer-
Canada Balsam sets slowly; it may take months to dry to nonstickness. Although all Hematoxylin and Eosin (H&E) stained slides fade with storage, slides mounted in Canada Balsam may fade quicker than slides mounted with DPX Mountant for histology. Canada Balsam is also a poor preservative for basic aniline dyes and slides stained for Prussian Blue.
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