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40587

Millipore

Dichloran Glycerol Agar

suitable for microbiology, NutriSelect® Plus

Synonym(s):

DG 18 Agar

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About This Item

UNSPSC Code:
41171609
NACRES:
NA.85

Agency

according to ISO 21527-2:2008

Quality Level

200

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
chloramphenicol, 0.1 g/L
dextrose, 10.0 g/L
dichloran, 0.002 g/L
magnesium sulfate, 0.5 g/L
monopotassium phosphate, 1.0 g/L
peptic digest of animal tissue, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

5.6±0.2 (25 °C)

application(s)

bioburden testing
environmental
food and beverages
microbiology

suitability

selective for molds (General Media)
selective for yeasts (General Media)

General description

Dichloran Glycerol Medium Base with Chloramphenicol is recommended for selective isolation of xerophilic moulds from food samples. It is a medium for enumeration of xerophilic fungi from low-moisture foods.

Application

Dichloran Glycerol Agar has been used for culturing Aspergillus flavus AF57 on rice grains and fungal populations from conidial suspension treated rice grains.

Preparation Note

Suspend 15.8 grams in 500 ml distilled water. Heat to boiling to dissolve the medium completely. Add 110 grams of glycerol (Analytical Reagent Grade). Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL7506
BCCF6085
BCCF3671
BCCF2834
BCCD5552

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A D Hocking et al.
Applied and environmental microbiology, 39(3), 488-492 (1980-03-01)
A low water activity (alpha omega) medium (0.95 alpha omega) containing 18% (wt/wt) glycerol and 2 micrograms of dichloran per ml was developed for enumerating the fungal flora of dried and semidried foods. The medium, designated DG18, was shown to
Biocontrol activity of volatile-producing Bacillus megaterium and Pseudomonas protegens against Aspergillus flavus and aflatoxin production on stored rice grains
Mannaa M, et al.
Mycobiology, 45(3), 213-219 (2017)
Effect of Temperature and Relative Humidity on Growth of Aspergillus and Penicillium spp. and Biocontrol Activity of Pseudomonas protegens AS15 against Aflatoxigenic Aspergillus flavus in Stored Rice Grains
Mannaa M and Kim KD
Mycobiology, 46(3), 287-295 (2018)

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