NGLYF-RO

N-Glycosidase F

PNGase F of Flavobacterium meningosepticum, recombinant from E. coli

• Synonym(s): glycosidase
• UNSPSC Code: 12352204
• EC Number: 3.5.1.52 (BRENDA, IUBMB)
• NACRES: NA.54
• Specific activity: ~25000 units/mg protein
• Assay: ≥90% (SDS-PAGE)
• Recombinant: expressed in E. coli

Properties

• recombinant: expressed in E. coli
• conjugate: (N-linked)
• assay: ≥90% (SDS-PAGE)
• form: solution
• specific activity: ~25000 units/mg protein
• packaging: pkg of 0.1 mL (11365169001 [100 U]), pkg of 0.25 mL (11365177001 [250 U])
• manufacturer/tradename: Roche
• optimum pH: 7.0-8.0
• storage temp.: −20°C

Description

General description

Peptide-N-glycosidase F, Peptide-N⁴-(acetyl-β-glucosaminyl)-asparagine amidase

Application

Use N-glycosidase F to cleave all types of asparagine-bound N-glycans, provided that the amino group as well as the carboxyl group are present in a peptide linkage, and that the oligosaccharide has the minimum length of the chitobiose core unit. The reaction products are ammonia, aspartic acid (in the peptide chain), and the complete oligosaccharide.
Note: N-Glycosidase F, recombinant is also available as a lyophilizate without glycerol.

Physical form

Solution in 50 mM sodium phosphate, 12.5 mM EDTA, 50% glycerol (v/v), pH 7.2

Other Notes

For life science research only. Not for use in diagnostic procedures.

One unit is the enzyme activity which hydrolyzes 1 nmol dabsyl fibrin glycopeptide or 0.2 nmol dansyl fetuin glycopeptide within 1 minute at 37 °C at pH 7.8.

Legal Information

The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: No data available
• flash_point_c: No data available