MABS1330
Anti-N1-Phosphohistidine (1-pHis) Antibody, clone SC1-1
clone SC1-1, from rabbit
Synonym(s):
N1-Phosphohistidine
About This Item
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
SC1-1, monoclonal
species reactivity
E. coli, human
species reactivity (predicted by homology)
all
technique(s)
affinity chromatography: suitable
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
phosphorylation (N1-pHis)
General description
Specificity
Immunogen
Application
Western Blotting Analysis: A representative lot detected heat-sensitive histidine N1-phosphorylation (1-pHis) in multiple cell lysates (Fuhs, S.R., et al. (2015). Cell. 162(1):198-210).
Immunocytochemistry Analysis: A representative lot detected N1-phosphohistidine (1-pHis) immunoreactivity distinct from that of 3-pHis in 4% paraformaldehyde-fixed HeLa cells and murine bone marrow-derived macrophages by fluorescent immunocytochemistry. The 1-pHis immunoreactivity was found in regions surrounding acidic compartments, but not inside these compartments or nuclei (Fuhs, S.R., et al. (2015). Cell. 162(1):198-210).
Immunoaffinity Purification: A representative lot was cross-linked to protein A resins for immunoaffinity purification of 1-pHis proteins from cell lysates prior to LC-MS/MS analysis (Fuhs, S.R., et al. (2015). Cell. 162(1):198-210).
Note: DO NOT HEAT SAMPLES prior to phosphohistidine detection. Histidine phosphorylation is heat and acid labile. To generate negative control for specificity test, an aliquot of sample can be heated at 95ºC for 10-15 minutes to reverse histidine phosphorylation. Alternatively, an aliquot of sample can be incubated under acidified pH at 37ºC for 15 minutes to reduce histidine phosphorylation. Acidify each 100 µL sample with 25 µL of 1 M HCl before the incubation, then neutralize with 25 µL of 1 M NaOH prior to phosphohistidine detection.
Quality
Western Blotting Analysis: 0.3 µg/mL of this antibody detected recombinant human NME1 (NM23-H1) with N1-phosphohistidine (1-pHis) in a 5 µg aliquot of autophosphorylation reaction.
Target description
Physical form
Other Notes
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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