MABF147B

Anti-CD45R (B220) (human/mouse), redFluor^® 710, clone RA3-6B2 Antibody

clone RA3-6B2, from rat, redFluor^® 710

• Synonym(s): Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), Lymphocyte antigen 5 (Ly-5), T200, CD45, CD45R (B220)
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• conjugate: redFluor^® 710
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: RA3-6B2, monoclonal
• species reactivity: mouse, human
• packaging: antibody small pack of 25 μg
• technique(s): flow cytometry: suitable
• UniProt accession no.: P06800
• target post-translational modification: unmodified
• Gene Information: human ... PTPRC(5788) ; mouse ... Ptprc(19264)

Description

General description

The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Anti-CD45R (B220) (human/mouse), redFluor^® 710, clone RA3-6B2 Antibody detects level of CD45R and has been published and validated for use in Flow Cytometry.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD45R (B220) in one million C57Bl/6 splenocytes.

Physical form

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.

Purified by size-exclusion chromatography

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

redFluor is a registered trademark of Tonbo Biotechnologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable