Skip to Content
Merck
All Photos(1)

Key Documents

MAB2575

Sigma-Aldrich

Anti-Integrin α5β1 Antibody, clone BMC5

ascites fluid, clone BMC5, Chemicon®

Synonym(s):

VLA-5

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

BMC5, monoclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG2b

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ITGA5(3678)

Specificity

Antibody MAB2575 was raised against mouse integrin VLA-5 (alpha5beta1), a fibronectin receptor. The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells. Specificity of the antibody was verified by its ability to immunoprecipitate alpha5beta1 heterodimers from (125)-I-surface labeled cells, reciprocal depletion of VLA-5 antigen from cell lysate with antiserum against the cytoplasmic domain of a5 integrin subunit, and immunoprecipitation of VLA-5 integrin from cells known to express this integrin. Cell lines tested to express alpha5beta1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes. Characterization by immunoprecipitation was typically analyzed by SDS-PAGE using 6% gels under non-reducing conditions.

Immunogen

Mouse embryonal carcinoma P19 cells.

Application

Detect Integrin α5β1 using this Anti-Integrin α5β1 Antibody, clone BMC5 validated for use in FC, IP, IH.
Inhibition of cell adhesion to fibronectin: 10 μL/mL

Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMC5 alone may not be complete.

Immunoprecipitation from lysate of 10E6 cell equivalent: 2 μL

Flow cytometry: 10μL/mL

Immunohistochemistry on acetone-fixed frozen sections only; formalin fixation is not recommended.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins

Physical form

Liquid ascites.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Integrin adhesion and force coupling are independently regulated by localized PtdIns(4,5)2 synthesis.
Legate, KR; Takahashi, S; Bonakdar, N; Fabry, B; Boettiger, D; Zent, R; Fassler, R
The Embo Journal null
Alaknanda Emery et al.
Frontiers in cell and developmental biology, 11, 1261038-1261038 (2023-11-09)
The ovarian follicle is a complex structure that protects and helps in the maturation of the oocyte, and then releases it through the controlled molecular and structural remodeling process of ovulation. The progesterone receptor (PGR) has been shown to be
Vinay Bhaskar et al.
Journal of translational medicine, 5, 61-61 (2007-11-29)
Integrins are important adhesion molecules that regulate tumor and endothelial cell survival, proliferation and migration. The integrin alpha5beta1 has been shown to play a critical role during angiogenesis. An inhibitor of this integrin, volociximab (M200), inhibits endothelial cell growth and
Georgina P Coló et al.
Journal of cell science, 136(9) (2023-05-02)
Fibronectin (FN)-binding integrins control a variety of cellular responses through Rho GTPases. The FN-binding integrins, αvβ3 and α5β1, are known to induce different effects on cell morphology and motility. Here, we report that FN-bound αvβ3 integrin, but not FN-bound α5β1
Mitasha Bharadwaj et al.
Nature communications, 8, 14348-14348 (2017-01-28)
Upon binding to the extracellular matrix protein, fibronectin, αV-class and α5β1 integrins trigger the recruitment of large protein assemblies and strengthen cell adhesion. Both integrin classes have been functionally specified, however their specific roles in immediate phases of cell attachment

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service