AB5539 is the anti Anti-Neurofilament H (200 kDa) Antibody. I does stain the cytoplasmic filaments in neuronal cell bodies, and especially axons. In my opinion, most likely the weaker and stonger staining pattern is due to the NF antibody reacting to phosphorylated epitope in the axon. You may wish to review genral literature on this line, e.g. literature PMID: 3133402, PMID: 6416876,
PMID: 2998843
AB5539
Anti-Neurofilament H (200 kDa) Antibody
CHEMICON®, chicken polyclonal
Synonym(s):
NF-H
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
rat, pig, bovine, feline, human, mouse
Application:
ICC, IHC (p), WB
Citations:
192
Pricing and availability is not currently available.
Product Name
Anti-Neurofilament H Antibody, Chemicon®, from chicken
biological source
chicken
Quality Segment
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, pig, bovine, feline, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
bovine ... Nefh(528842)
cat ... Nefh(101087272)
human ... NEFH(4744)
mouse ... Nefh(380684)
pig ... Nefh(100156492)
rat ... Nefh(24587)
General description
200 kDa
Immunogen
Purified bovine NF-H.
Application
Anti-Neurofilament H Antibody is an antibody against Neurofilament for use in IC, IH, IH(P) & WB.
Western blot: 1:1,000-1:10,000 using ECL. Recognizes a band of 200-220 kDa.
Immunohistochemistry: 1:1,000-1:10,000 on frozen or paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde.
Immunocytochemistry: 1:1,000-1:10,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:1,000-1:10,000 on frozen or paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde.
Immunocytochemistry: 1:1,000-1:10,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes Neurofilament-Heavy (NF-H). The antibody stains sharply defined cytoplasmic filaments in neuronal cell bodies and especially axons.
Physical form
Format: Purified
Purified chicken IgY in PBS containing 5mM sodium azide.
Analysis Note
Control
Positive control: brain.
Negative control: kidney, liver.
Positive control: brain.
Negative control: kidney, liver.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
WGK
WGK 2
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I am staining FFPE postmortem human spinal cord tissues. What does it mean if some motor neuron cell bodies have intense NF-H signal? Also should I interpret weak signals in the white and grey matter as background? Dendrites? Degenerating axons?
1 answer-
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Does this antibody detect phosphorylated or non-phosphorylated NFH or both?
1 answer-
This product is a polyclonal antibody that was made against biochemically isolated NF-H purified from bovine spinal cord. This preparation is dominated by axonal forms of NF-H which are heavily phosphorylated on the multiply repeated NF-H KSP type sequences, and this antibody reacts very strongly with these phosphorylated repeats. Reactivity with non-phosphorylated KSP sequences is orders of magnitude weaker. In most species there is some cross-reactivity with the phosphorylated KSP sequences found in the related neurofilament subunit NF-M which are similar but not identical to those of NF-H. The antibody recognizes phosphorylated NF-H strongly in all mammals tested to date.
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