A molecular biology grade is suggested as the reagent is quality guaranteed to ensure it is RNAse free. An item of interest would be product E7889. This is a ~0.5 M EDTA solution with a pH that will be within the range of 7.5 - 8.5. It is suitable for molecular biology as no DNases, RNases, Nickase, or proteases are detected in this reagent. The lot specific certificate of analysis should be reviewed for this product to determine the specific concentration/pH that the item was released with. Upon verification of these details, this item can be diluted to 100 mM with the appropriate pH adjustment using RNase free reagents.
324504
EDTA
ULTROL® Grade, 500 mM Solution, pH 8.0
Synonym(s):
EDTA, 500 mM Solution, pH 8.0, ULTROL® Grade
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About This Item
Empirical Formula (Hill Notation):
C10H16N2O8
CAS Number:
Molecular Weight:
292.24
MDL number:
UNSPSC Code:
12352107
NACRES:
NA.25
Pricing and availability is not currently available.
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description
Merck USA index - 14, 3517
Quality Level
form
solution
reaction suitability
reagent type: chelator
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
concentration
500 mM
color
colorless to pale yellow
pH
8
shipped in
ambient
storage temp.
10-30°C
SMILES string
N(CCN(CC(=O)O)CC(=O)O)(CC(=O)O)CC(=O)O
InChI
1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)
InChI key
KCXVZYZYPLLWCC-UHFFFAOYSA-N
General description
A useful agent for radioimmunoimaging.
Ethylenediaminetetraacetic acid (EDTA) is a metal chelating agent, which is used in treating patients with heavy-metal poisonings like lead or mercury. It is also used to minimize the non-specific serum protein interactions in enzyme immunoassays. Due to its ability to inhibit protein agglutination, it is useful to make blood/plasma samples and in immunoanalysis to detect CD3 cells. EDTA combines with buffers or ionic salts to stimulate the immune cells in the blood samples. It is used in multiple aspects to treat and analyze psoriasis. EDTA functions as an antioxidant to combat psoriasis, while it is also used to understand the disease at a molecular level.[1][2]
Application
EDTA, 500 mM Solution, pH 8.0, ULTROL® Grade has been used along with phosphate buffer saline (PBS) for cell disruption to harvest crypts in situ hybridization of nematodes.[3] It has also been used as a component of isoelectric focusing solution (IEF) to resuspend myofibrils for protein studies.[4]
Warning
Toxicity: Irritant (B)
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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I need to get 100 mM EDTA pH 6.5 for RNA work. I guess I could start with EDTA pH 8, but I need to make sure that it's RNase free. Which of your EDTA products are suitable for RNA work? Thank you
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