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228250

Cholesterol Oxidase, Streptomyces sp.

Cholesterol Oxidase, Streptomyces sp. CAS 9028-76-6, is a native cholesterol oxidase that catalyzes the oxidation of cholesterol to cholesterone and hydrogen peroxide.

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Synonym(s):

Cholesterol Oxidase, Streptomyces sp., Cholesterol:oxygen oxidoreductase

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About This Item

CAS Number:
EC Number:
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
Specific activity:
≥3 units/mg solid, ≥40 U/mg
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Quality Segment

form

lyophilized solid

specific activity

≥3 units/mg solid, ≥40 U/mg

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated, protect from light

color

yellow

solubility

water: 20 mg/mL

foreign activity

Catalase ≤1%, glucose oxidase and uricase ≤0.01%

shipped in

ambient

storage temp.

−20°C

General description

Native cholesterol oxidase from Streptomyces species. Catalyzes oxidation of cholesterol to cholesterone and hydrogen peroxide (isoelectric points: 6.1 and 7.3). Suitable for determination of total cholesterol when coupled with cholinesterase and peroxidase. Has an optimal pH of 6.0-8.0.

Other Notes

Gadda, G., et al. 1997. Eur. J. Biochem. 250, 369.
One unit is defined as the amount of enzyme that will oxidize 1.0 µmol of cholesterol per min at 25°C, pH 7.0.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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This Item
G1924C8868C5421
description

Cholesterol Oxidase, Streptomyces sp. CAS 9028-76-6, is a native cholesterol oxidase that catalyzes the oxidation of cholesterol to cholesterone and hydrogen peroxide.

description

recombinant, expressed in E. coli

description

lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli

description

aqueous solution, ≥30 units/mg protein (biuret)

specific activity

≥40 U/mg, ≥3 units/mg solid

specific activity

≥5 units/mg protein

specific activity

≥50 units/mg protein

specific activity

≥30 units/mg protein (biuret)

form

lyophilized solid

form

solid

form

lyophilized powder

form

aqueous solution

shipped in

ambient

shipped in

-

shipped in

-

shipped in

dry ice

solubility

water: 20 mg/mL

solubility

-

solubility

-

solubility

50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)

storage condition

OK to freeze, desiccated, protect from light

storage condition

-

storage condition

-

storage condition

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C


Pictograms

Health hazard

Signal Word

Danger

Hazard Codes

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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