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565320-U

Supelco

Ascentis® RP-Amide (3 µm) HPLC Columns

L × I.D. 5 cm × 4.6 mm, HPLC Column

Synonym(s):

RP-Amide Reversed-Phase Chromatography Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® RP-Amide HPLC Column, 3 μm particle size, L × I.D. 5 cm × 4.6 mm

material

stainless steel column

Quality Level

Agency

suitable for USP L60

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

extent of labeling

19.5% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

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General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.

Application


  • A validated UHPLC method for the determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts using an RP-Amide fused-core column.: This paper presents a validated UHPLC method employing an RP-Amide fused-core column for the accurate determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts, highlighting the column′s efficiency and robustness (Fibigr et al., 2018).

Features and Benefits

  • Excellent retention and peak shape for polar compounds
  • 100% aqueous compatibility
  • Ultra low bleed, LC-MS compatible
  • Unique selectivity

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 151, 291-300 (2018-02-08)
The presented work describes the development and validation of a rapid UHPLC-UV method using a fused core particle column with an RP-Amide stationary phase for the separation and quantitative analysis of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. Three
Rihana Parveen Shaik et al.
Journal of pharmaceutical analysis, 3(6), 489-499 (2013-12-01)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for simultaneous quantification of tolterodine and its metabolite 5-hydroxy methyl tolterodine in rat plasma. Tolterodine-d6 and 5-hydroxy methyl tolterodine-d14 were used as internal standards (IS). Chromatographic separation was performed on Ascentis Express

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