505013
Discovery® RP-Amide C16 (5 µm) HPLC Columns
L × I.D. 15 cm × 4.6 mm, HPLC Column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
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product name
Discovery® RP-AmideC16 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Agency
suitable for USP L60
feature
endcapped
manufacturer/tradename
Discovery®
extent of labeling
11% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
15 cm × 4.6 mm
surface area
200 m2/g
surface coverage
2.6 μmol/m2
impurities
<10 ppm metals
matrix
silica gel, high purity, spherical base material
matrix active group
amide, alkyl phase
particle size
5 μm
pore size
180 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
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General description
Discovery® RP-AmideC16 HPLC Column consists of a stationary phase made up of high-purity C16-silica that includes a polar-embedded amide function attached to the silica surface with a propyl group.
Application
Discovery RP-AmideC16 HPLC Column may be used for the separation of:
- Impurities in drugs such as closantel, levamisole and azaconazole during their determination by high performance liquid chromatography-diode array detection (HPLC-DAD).
- Vitamin B6 compounds in food samples during their determination using LC coupled with fluorescence detection (FLD).
- Vitamin C and other fat soluble vitamins in mature seeds of Lathyrus L. taxa during their identification using HPLC with ultraviolet (UV) detection.
- Zidovudine in combination with other pharmaceuticals in intravenous fluid mixtures during the determination using HPLC combined with photodiode array (PDA) detector.
Discovery® RP-AmideC16 HPLC column may be used for the separation of:
- Phenolic compounds, purine alkaloids and theanine in Camellia species by high performance liquid chromatography (HPLC) equipped with photodiode array detector (PDA).
- Proprietary pharmaceutical compounds using HPLC combined with computer-assisted screening and Plackett−Burman experimental design.
- Hydrophilic bases by LC with principal component analysis (PCA).
- 2,4-Dinitrophenylhydrazone derivatives by HPLC equipped with ultraviolet (UV) spectrometric detection.
Features and Benefits
- Excellent retention and resolution of polar compounds
- Unique selectivity compared to C18
- Excellent peak shape and efficiency
- Less hydrophobic than C18 columns
- Compatible with 100% aqueous mobile phases
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Determination of vitamin B6 compounds in foods using liquid chromatography with post-column derivatization fluorescence detection.
Vinas P, et al.
Chromatographia, 59(5-6), 381-386 (2004)
An improved HPLC method for simultaneous determination of phenolic compounds, purine alkaloids and theanine in Camellia species
Peng L, et al.
J. Food Compos. Anal., 21(7), 559-563 (2008)
Strategy for developing and optimizing liquid chromatography methods in pharmaceutical development using computer-assisted screening and Plackett-Burman experimental design
Li W and Rasmussen HT
Journal of Chromatography A, 1016(2), 165-180 (2003)
Vitamin and fatty acid contents in seeds of some taxa belonging to genus Lathyrus L. growing in Turkey.
Sahin A, et al.
Acta Botanica Gallica, 156(3), 331-339 (2009)
Isomerization of aldehyde-2, 4-dinitrophenylhydrazone derivatives and validation of high-performance liquid chromatographic analysis
Uchiyama S, et al.
Journal of Chromatography A, 996(1-2), 95-102 (2003)
Protocols
Separations of Tetracycline Antibiotics by Reversed Phase HPLC, Using Discovery Columns
Chromatograms
application for HPLCapplication for SPE, application for HPLCapplication for HPLCOur team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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