MBD0032

Eubacteria FISH probe - ATTO488

Probe for fluorescence in situ hybridization (FISH), 20 µM in water

• Synonym(s): Fluorescent Probe
• UNSPSC Code: 12352200
• NACRES: NA.55

Properties

• Quality Level: 200
• technique(s): FISH: suitable
• fluorescence: λ_ex 504 nm; λ_em 521 nm (ATTO488)
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Fluorescent In Situ Hybridization technique (FISH) is based on the hybridization of fluorescent labeled oligonucleotide probe to a specific complementary DNA or RNA sequence in whole and intact cells. Microbial FISH allows the visualization, identification and isolation of bacteria due to recognition of ribosomal RNA also in unculturable samples.

FISH technique can serve as a powerful tool in the microbiome research field by allowing the observation of native microbial populations in diverse microbiome environments, such as samples from human origin (blood and tissue), microbial ecology (solid biofilms and aquatic systems) and plants.

Prokaryotic single cell life forms are divided into two domains, called Bacteria and Archaea, originally categorized as Eubacteria and Archaebacteria. However both terms, Eubacteria and Bacteria are still being used in microbiology. Eubacteria probe recognizes most bacteria as it is complementary to a portion of 16S rRNA found in almost all bacteria.^,
FISH technique was successfully used to identify different bacteria with the universal bacterial probe in various samples such as, pure culture (as described in the figure legends), blood cultures^,, periapical tooth lesions¹², saliva¹³, biofilms from voice prostheses¹⁴, subgingival biofilm¹⁵, aortic wall tissue¹⁶, buccal epithelial cells, pure culture and cell culture¹⁷, intestine tissue embedded in paraffin¹⁸, necrotizing fasciitis and pure culture¹⁹, colon sections embedded in paraffin^20,21, cancer tissues^22,23, environmental samples²⁴ and gut of the medicinal leech²⁵. The probe can also be used for combined technique of FISH and Flow cytometric analysis. ^9,26,27

It is strongly recommended to include positive and negative controls in FISH assays to ensure specific binding of the probe of interest and appropriate protocol conditions. We offer positive (MBD0032/33) and negative (MBD0034/35) control probes, that accompany the specific probe of interest.

Application

Eubacteria FISH probe - ATTO488 is suitable to use as a probe for fluorescence in situ hybridization (FISH) to recognize Eubacteria cells .

Features and Benefits

• Visualize, identify and isolate bacteria cells.
• Observe native bacteria cell populations in diverse microbiome environments.
• Specific, sensitive and robust identification of bacteria cells in mixed microorganism population.
• Specific, sensitive and robust identification even when bacteria are in low abundance in the sample.
• FISH can complete PCR based detection methods by avoiding contaminant bacteria detection.
• Provides information on bacteria morphology and allows to study biofilm architecture.
• Identify various bacteria in environmental and clinical samples such as, formalin-fixed paraffin-embedded (FFPE) samples, blood cultures, saliva and more.
• The ability to detect bacteria in its natural habitat is an essential tool for studying host-microbiome interaction.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable