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M7167

Sigma-Aldrich

M2 medium

With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture

Synonym(s):

Cell Culture M2, Cell Culture Medium, M2 Medium Solution

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About This Item

MDL number:
MFCD00283761
UNSPSC Code:
12352207
NACRES:
NA.75

sterility

sterile-filtered

form

liquid

storage condition

protect from light

technique(s)

cell culture | embryo: suitable

impurities

endotoxin, tested

components

HEPES: 5.42726 g/L
NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L

shipped in

ambient

storage temp.

2-8°C

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Application

Recommended for manipulation of mouse embryos at ambient temperature. M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.

M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator.

Quality

Presence of particulates in solution will not affect product′s performance.

Reconstitution

Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.

Analysis Note

This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Gene expression patterns : GEP, 25-26, 46-58 (2017-06-07)
Cumulus cells (CCs) are considered as an important source to predict oocyte quality. Despite numerous candidate genes in CCs have been identified for embryonic developmental competence, the results are inconsistent. The next generation RNA-sequencing was used to investigate the transcriptomic
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