M4276
Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse
clone MY-32, ascites fluid
Synonym(s):
Anti-Myosin Antibody
Sign Into View Organizational & Contract Pricing
All Photos(3)
About This Item
Recommended Products
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
MY-32, monoclonal
contains
15 mM sodium azide
species reactivity
rat, chicken, rabbit, mouse, human, bovine, guinea pig, feline
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using skeletal muscle tissue
indirect immunofluorescence: 1:400
western blot: 1:1,000 using rabbit leg muscle extract
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MYH1(4619) , MYH2(4620)
mouse ... Myh1(17879) , Myh2(17882)
rat ... Myh1(287408) , Myh2(691644)
Looking for similar products? Visit Product Comparison Guide
General description
Localizes an epitope on the myosin heavy chain. Stains the fast (type II) and neonatal isomyosin molecules found in skeletal muscle, but does not stain cardiac muscle, smooth muscle or non-muscle myosin in cultured cells. Does react with human rhabdomyosarcomas.
Monoclonal Anti-Skeletal Myosin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Myosin is a 480,000 dalton protein known to interact with actin in muscle and in non-muscle cells. It contains two identical heavy chains (200,000 daltons each) and four light chains (15,000-26,000 daltons). Myosin molecules consist of two major regions: tails (rods) and heads; they aggregate into filaments through the tail region and interact with actin and with ATP through the head region. Multiple forms of myosin heavy chains exist for each muscle type-skeletal, cardiac, smooth and non-muscle isomyosin forms exist in different types of skeletal muscle, depending on the physiological function of the muscle. These are designated at type I (slow twitch) and type II (fast-twitch). Type II fibers can be further subdivided in types IIA, IIB, and IIC.
Specificity
Monoclonal Anti-Skeletal Myosin is specific for the myosin heavy chain. It does not stain human or animal cardiac or smooth muscle myosin or cells grown by tissue culture (nonmuscle myosin). It has been demonstrated on human skeletal muscle that the antibody stains the fast twitch (type II) isomyosin molecules. Monoclonal Anti-Skeletal Myosin antibody does react with human rhabdomyosarcomas.
Immunogen
rabbit muscle myosin.
Application
Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse has been used in:
- immunohistochemistry
- immunostaining
- western blotting at a dilution 1:1000 and 1:90000†
- indirect immunofluorescence (dilution 1:400) of formalin-fixed, paraffin-embedded sections of human or animal skeletal muscle tissue preparation.
- dot immunobinding on muscle extracts or purified myosin preparations
The level of mysosin (fast) in serum samples from sportsmen with past injury was determined by western blot using monoclonal mouse anti-myosin (skeletal/fast) as the primary antibody at a dilution of 1:90000.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Contractility and kinetics of human fetal and human adult skeletal muscle
Racca AW, et al.
The Journal of Physiology, 591(12), 3049-3061 (2013)
David Vaughan et al.
PloS one, 11(3), e0149046-e0149046 (2016-03-18)
A silencer region (I-allele) within intron 16 of the gene for the regulator of vascular perfusion, angiotensin-converting enzyme (ACE), is implicated in phenotypic variation of aerobic fitness and the development of type II diabetes. We hypothesised that the reportedly lower
Shanshan Wang et al.
Frontiers in cell and developmental biology, 8, 618617-618617 (2021-01-30)
Enhancer of zeste homolog 2 (EZH2) is the catalytic subunit of polycomb repressive complex 2 and contains a SET domain that catalyzes histone H3 trimethylation on lysine 27 (H3K27me3) to generate an epigenetic silencing mark. EZH2 interacts with transcription factors
An optimized histochemical method to assess skeletal muscle glycogen and lipid stores reveals two metabolically distinct populations of type I muscle fibers
Prats C, et al.
PLoS ONE, 8(10), e77774-e77774 (2013)
Charlotte Suetta et al.
PloS one, 7(12), e51238-e51238 (2013-01-04)
Important insights concerning the molecular basis of skeletal muscle disuse-atrophy and aging related muscle loss have been obtained in cell culture and animal models, but these regulatory signaling pathways have not previously been studied in aging human muscle. In the
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service