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Minimum Essential Medium Eagle

Name: Minimum Essential Medium Eagle
Brand: SIGMA

Alpha Modification, with L-glutamine, ribonucleosides and deoxyribonucleosides, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

MEM

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

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Properties

Quality Segment

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO₃: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

shipped in

ambient

storage temp.

2-8°C

Description

General description

Minimum Essential Medium (MEM) is a synthetic cell culture media developed by Harry Eagle. This medium has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to this formulation that incorporates either Hanks′ or Earle′s salts has broadened the usage of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.

Application

Minimum essential medium Eagle has been used to:

maintain natural killer (NK) cell lines
culture Leishmania donovani strains
culture epidermal neural crest stem cells (EPI-NCSCs) from the bulge of rat hair follicles

Preparation Note

Formulated to contain 10.1 grams of powder per liter of medium.

Supplement with 2.2 g/L sodium bicarbonate.

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This Item	M0894	FG0325	M0769
Sigma-Aldrich M0644 Minimum Essential Medium Eagle	Sigma-Aldrich M0894 Minimum Essential Medium Eagle	SAFC FG0325 Minimum Essential Medium Eagle	SAFC M0769 Minimum Essential Medium Eagle
description Alpha Modification, with L-glutamine, ribonucleosides and deoxyribonucleosides, without sodium bicarbonate, powder, suitable for cell culture	description Alpha Modification, with L-glutamine and sodium pyruvate, without ribonucleosides, deoxyribonucleosides and sodium bicarbonate, powder, suitable for cell culture	description With Earle′s salts, L-alanyl-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture	description AutoMod^™, with Earle′s salts, without L-glutamine and sodium bicarbonate, powder, suitable for cell culture
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form powder	form powder	form liquid	form powder
components NaHCO₃: no L-glutamine: yes phenol red: yes HEPES: no sodium pyruvate: yes	components stable glutamine: no Hanks’ salts (2% CO₂): no sodium pyruvate: yes NaHCO₃: no phenol red: yes glucose: yes L-glutamine: yes Earle’s salts (5% CO₂): yes HEPES: no	components stable glutamine: yes L-glutamine: no phenol red: yes sodium pyruvate: no Earle’s salts (5% CO₂): yes HEPES: no Hanks’ salts (2% CO₂): no glucose: yes NaHCO₃: yes	components NaHCO₃: no phenol red: yes Earle’s salts (5% CO₂): yes L-glutamine: no sodium pyruvate: no HEPES: no
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
Quality Level 500	Quality Level 500	Quality Level 400	Quality Level 500

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Pictograms

GHS07

Signal Word

Warning

Hazard Codes

H317

Precautionary Statements

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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