Skip to Content
Merck
All Photos(1)

Key Documents

I1161

Sigma-Aldrich

ImmunoHistoMount™

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12171500
NACRES:
NA.47
Pricing and availability is not currently available.

application(s)

hematology
histology

Quality Level

100

storage temp.

room temp

Related Categories

Application

ImmunoHistoMount™ is a permanent aqueous mounting medium designed for tissue sections and cell smears with peroxidase and alkaline phosphatase chromogens that cannot be dehydrated with organic solvents.[1][2][3] This mounting medium preserves fast red, aminoethylycarbazole (AEC), BCIP/NBT, and BCIP/INT chromogens and is compatible with counterstains like hematoxylin and nuclear fast red (NFR). ImmunoHistoMount is also suitable for chromogens like DAB and DAB with nickel and cobalt. It is not compatible with routine H & E staining.

Linkage

ImmunoHistoMount™ is an aqueous-based mounting medium comparable to Crystal Mount™

Legal Information

Crystal Mount is a trademark of Biomeda Corp.
ImmunoHistoMount is a trademark of ImmunoBioScience Corp.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
MKCX3276
MKCW0175
MKCT4569
MKCS6671
MKCQ7057

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Therapeutic immunization and local low-dose tumor irradiation, a reinforcing combination.
Draghiciu O, et al.
International Journal of Cancer. Journal International Du Cancer, 134(4), 859-872 (2014)
Seizure frequency correlates with loss of dentate gyrus GABAergic neurons in a mouse model of temporal lobe epilepsy.
Buckmaster P S, et al.
The Journal of Comparative Neurology, 525(11), 2592-2610 (2017)
The effect of moving point of contact stimulation on chondrocyte gene expression and localization in tissue engineered constructs.
Kaupp J A, et al.
Annals of Biomedical Engineering, 41(6), 1106-1119 (2013)
Fernanda Medina-Flores et al.
Brain, behavior, and immunity, 89, 118-132 (2020-06-03)
Sleep loss in the rat increases blood-brain barrier permeability to circulating molecules by disrupting interendothelial tight junctions. Despite the description of the ultrastructure of cerebral microvessels and the evidence of an apparent pericyte detachment from capillary wall in sleep restricted
Damon T Jacobs et al.
Disease models & mechanisms, 9(7), 789-798 (2016-08-03)
Primary cilia extend from the plasma membrane of most vertebrate cells and mediate signaling pathways. Ciliary dysfunction underlies ciliopathies, which are genetic syndromes that manifest multiple clinical features, including renal cystic disease and obesity. THM1 (also termed TTC21B or IFT139)

Questions

1–9 of 9 Questions  

  1. Can Immunohistomount (I1161) be used to coverslip IHC slides stained with DAB?

    1 answer

    1. Immunohistomount (I1161) is suitable for mounting IHC slides stained using DAB as the chromogen. Unlike Fast Red or AEC, which are alcohol-soluble, DAB is not soluble in either alcohol or in clearing agents such as xylene or xylene substitutes. Most other permanent mounting medias are also suitable for use, such as DPX, NeoMount, Entellen, Eukit, and Permount.

      Helpful?

  2. ImmunoHistoMount (Product No. I1161) is stated to be an aqueous mounting media.  Will slides quickly fade?

    1 answer

    1. ImmunoHistoMount is considered a permanent aqueous mounting media.  Although all slides will eventually fade, slides coverslipped with ImmunoHistoMount should maintain staining for a relatively long period of time.

      Helpful?

  3. What is the step-by-step procedure for using ImmunHistoMount (Product No. I1161)?

    1 answer

    1. 1. Bring the vial to room temperature.  2. Rinse slide to be mounted with Distilled or Deionized water, then touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface. 3. Turn the vial upside down and open the dropper to remove any air bubbles. 4. Apply 3-4 drops of mounting medium directly on top of the specimen and spread out evenly by tilting the slide back and forth to spread evenly, or use a 0.2 ml plastic pipette tip to evenly distribute the mounting media, making sure the tissue is not touched. Excess medium can be removed by touching the edges of the slide against a paper towel. 5. Let stand at room temperature for about 1 to 2 hours. Alternatively, the slides can be heated at 37-40°C for 40-60 minutes. After setting, the slides are ready for visualization under a microscope.

      Helpful?

  4. Can slides to be coverslipped with ImmunoHistoMount (Product No. I1161) be processed through organic solvents?

    1 answer

    1. Slides to be coverslipped with ImmunHistoMount cannot be dehydrated through organic solvents.

      Helpful?

  5. At what thickness should the slides be cut for use with ImmunoHistoMount (Product No. I1161)?

    1 answer

    1. Typically slides are cut at between 5 to 8 microns.

      Helpful?

  6. What are the suggested way to view slides that have been mounted with ImmunoHistoMount (Product No. I1161) under an oil immersion lens?

    1 answer

    1. There are two ways to look at slides under an oil immersion lens: 1. For oil immersion lens, a drop of oil can be placed directly on dried ImmunoHistoMount mounting medium for visualization. After use, the oil can be removed by wiping with Kimwipes. 2. Apply 2-3 drops of any organic mounting medium on top of dried ImmunoHistoMount mounting medium; which has protected the specimen, coverslip slide with coverslip; the slide is ready for visualization.

      Helpful?

  7. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  9. Is ImmunoHistoMount (Product No. I1161) compatible with slides stained with hematoxylin and eosin?

    1 answer

    1. Slides stained with hematoxylin and eosin should not be coverslipped with ImmunoHistoMount.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service