Unfortunately, the purity of this product is not determined by test specifications.
H3884
Hyaluronidase from bovine testes
Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid
Synonym(s):
Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.
Recommended Products
biological source
bovine testis
Quality Level
conjugate
conjugate (Glucosaminoglycan)
type
Type IV-S
form
lyophilized powder (essentially salt-free)
specific activity
750-3000 units/mg solid
mol wt
~55 kDa (four subunits of 14 kDa each)
application(s)
diagnostic assay manufacturing
relevant disease(s)
cancer
storage temp.
−20°C
Gene Information
cow ... HYAL1(515397) , HYAL2(281838)
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Related Categories
Biochem/physiol Actions
Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.
These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
Unit Definition
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Articles
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Protocols
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
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How pure is the protein?
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Can stock solutions of Product H3884, Hyaluronidase from bovine testes, be stored frozen after reconstitution?
1 answer-
Although Sigma-Aldrich has not tested the stability of stock solutions of Product H3884, Hyaluronidase from bovine testes, several publications cite storage of solutions of H3884 at -20 °C:1. Campbell, Keith H.S, "Transgenic Sheep form Cultured Cells", from Transgenesis Techniques: Principles and Protocols, Methods Mol. Biol., 180, 289-301 (2002). A 1500 IU/mL stock solution was stored in 20 mL aliquots at -20 °C.2. Marschall, S. and de Angelis, M.H., "In Vitro Fertilization/Cryopreservation", from Transgenic Mouse: Methods and Protocols, Methods in Molecular Biology, 209, pp. 35-50. Humana Press (2003). 1 mg/mL stock solutions in M2 medium or water, sterile-filtered (0.22 mm filter) can be stored in aliquots at -20 °C.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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