GE17-3717-51

HiTrap^® Capto^™ S ImpAct 5x1mL

Cytiva 17-3717-51, pack of 5x1ml

• Synonym(s): HITRAP^® CAPTO^™ S IMPACT
• UNSPSC Code: 23151817
• NACRES: NA.56

Properties

• ligand: (-SO₃)
• form: prepacked column
• crosslinking: cross-linked
• manufacturer/tradename: Cytiva 17-3717-51
• storage condition: (0.2 M sodium acetate in 20% ethanol)
• parameter: (Flow velocity: 220 - 600 cm/h); -44 psi max. pressure; 1-4 mL/min flow rate
• technique(s): liquid chromatography (LC): suitable
• matrix: high-flow agarose
• matrix active group: Polymeric Pyrrolidone and Sulfonate
• avg. part. size: 50 μm (d50V)
• cleaning in place: 4-12
• working range: 3-14
• capacity: binding capacity(>85 mg BSA/mL medium, >90 mg lysozyme/ml medium, >100 mg IgG/ ml medium)
• separation technique: cation exchange

Description

General description

HiTrap Capto S ImpAct (1 ml and 5 ml) are prepacked columns for small scale purifications, as well as optimization of methods and parameters, such as sample load and binding conditions. HiTrap Capto S ImpAct columns provide fast, reproducible and easy separations in a convenient format. The columns are best utilized when they are connected to liquid chromatography systems such as ÄKTA systems.

Capto S ImpAct is a BioProcess chromatography medium developed for large-scale purification of biopharmaceutical proteins. Based on the well-established Capto base matrix, it provides many opportunities for improved productivity and straightforward process development. This chromatography medium (resin) is available in a range of different bulk pack sizes and convenient prepacked formats for easy scale-up and process development. As a member of the BioProcess media range, Capto S ImpAct meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

HiTrap Capto S ImpAct columns are prepacked with Capto S ImpAct cation exchange chromatography medium for screening and small-scale protein purifications using ion exchange chromatography (IEX).

• Convenient prepacked column for fast, reproducible, and easy small-scale purifications.
• High binding capacity, > 100 mg MAb/mL medium.
• Efficient aggregate removal at high load of monoclonal antibodies.
• High-resolution intermediate purification and polishing based on the well-established Capto platform.
• Flexibility of design thanks to a large operational window of flow rates and bed heights for easy optimization and scaling.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Capto is a trademark of Cytiva

HiTrap is a registered trademark of Cytiva

Safety Information

• Storage Class Code: 3 - Flammable liquids