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F5662

Sigma-Aldrich

Monoclonal Anti-HLA Class I Antigen−FITC antibody produced in mouse

clone W6/32, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-HLA Class I Antigen

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

W6/32, monoclonal

form

buffered aqueous solution

species reactivity

monkey, human

technique(s)

direct immunofluorescence: 1:10 using acetone-fixed human tonsil frozen sections.
flow cytometry: 10 μL using 1 × 106 cells

isotype

IgG2a

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

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General description

Monoclonal Anti-Human HLA Class I Antigen (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from BALB/c mice immunized with human tonsil leukocyte membrane preparation. Class I proteins are highly polymorphic. HLA-A, -B, -C are classical class I proteins and are heterodimers. HLA-A consists of a polymorphic transmembrane α heavy chain and an invariant water-soluble light chain.
The function of HLA class I molecules is to present antigen to CD8+ lymphocytes. They are involved in the regulation of positive and negative selection of CD8+ T cells by thymic epithelial cells during T cell maturation. These molecules are important in allograft rejection and in the linkage of certain haplotypes in autoimmune diseases. Expression of HLA class I antigens may be modulated in pathological states. Malignant cells may lose these antigens while hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may display enhanced reactivity. Increased expression of these antigens is described in muscle cells affected by muscular dystrophy, inflammatory myopathy and other neuro-muscular disorders.

The previously assigned protein identifier P01892 has been merged into P04439. Full details can be found on the UniProt database.

Specificity

Recognizes the human HLA Class I antigen expressed on most human nucleated cells. The antibody reacts with a monomorphic epitope on the 44-45 kDa α-chain polypeptide of human class I HLA molecules (HLA-A,B,C). The antibody may be used in immunoprecipitation, complement mediated cytotoxicity, flow cytometry and immunohistochemical staining of acetone-fixed, frozen sections and cell smears. It cross-reacts with cells from African and Asian apes and old world monkeys. No reactivity is seen with cells from most new world monkeys or from non-primates. The epitope recognized by this antibody is sensitive to routine formalin fixation and paraffin embedding.
4th Workshop: code no. P1
5th Workshop: code nos. BP166, BP288, BP407, B 005

Immunogen

human tonsil leukocyte membrane preparation.

Application

FITC Monoclonal Anti-Human HLA Class I Antigen may be used for immunochemical analysis of HLA Class I antigens; Studies of HLA-A,B,C expression on embryonic and neoplastic tissues; and/or the purification of HLA-A,B,C antigens.
Monoclonal Anti-HLA Class I Antigen-FITC antibody produced in mouse has been used in:
  • flow cytometric analysis
  • immunoprecipitation
  • complement mediated cytotoxicity
  • immunocytochemistry

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M E Greenberg et al.
The EMBO journal, 17(10), 2777-2789 (1998-06-10)
Nef, a regulatory protein of human and simian immunodeficiency viruses, downregulates cell surface expression of both class I MHC and CD4 molecules in T cells by accelerating their endocytosis. Fibroblasts were used to study alterations in the traffic of class
Acellular amniotic membrane: an appropriate scaffold for fibroblast proliferation
Mahmoudi RM, et al.
Clinical and Experimental Dermatology, 38(6), 646-651 (2013)
Philipp Lennartz et al.
Biomedicines, 11(12) (2023-12-23)
The presence of circulating Hsp70 levels and their influence on the immunophenotype of circulating lymphocyte subsets were examined as diagnostic/prognostic biomarkers for the overall survival (OS) in patients with IDH-mutant WHO grade 3 oligodendroglioma, astrocytoma, and IDH-wildtype grade 4 glioblastoma
Lack of T cell response to iPSC-derived retinal pigment epithelial cells from HLA homozygous donors
Sugita S, et al.
Stem Cell Reports, 7(4), 619-634 (2016)
T L Chapman et al.
Immunity, 11(5), 603-613 (1999-12-11)
LIR-1 is a class I MHC receptor related to natural killer inhibitory receptors (KIRs). Binding of LIR-1 or KIRs to class I molecules results in inhibitory signals. Unlike individual KIRs, LIR-1 recognizes many class I alleles and also binds UL18

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