F4637

Anti-Human Polyvalent Immunoglobulins (whole molecule)−FITC antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Rabbit Anti-Human Polyvalent Immunoglobulins (whole molecule)−Fluorescein isothiocyanate
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00239694
• Conjugate: FITC conjugate
• Clone: polyclonal
• Application: IF (ANA), IF (d)
• Citations: 10

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: FITC conjugate
• antibody form: IgG fraction of antiserum
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• storage condition: protect from light
• technique(s): ANA-indirect immunofluorescence: 1:128, direct immunofluorescence: 1:80
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgA antibody is secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. IgM antibodies are present as pentamers in the serum and are produced in response to antigens
Purified human IgA, IgG and IgM are used to develop anti-sera in rabbits. The IgG fraction of each anti-sera is purified and conjugated to Fluorescein Isothiocyanate (FITC), in an alkaline reaction. Anti-Human Polyvalent Immunoglobulins (whole molecule)-FITC antibody specifically recognizes human IgA, IgG and IgM.

Immunogen

Purified human IgA, IgG, and IgM

Application

Anti-Human Polyvalent Immunoglobulins (whole molecule)-FITC antibody may be used for immunofluorescence of human peripheral blood lymphocytes at a working antibody dilution of 1:128. For anti-nuclear antibody assay of acetone-fixed rat liver cells, a minimum dilution of 1:128 is recommended. For direct labeling of human tonsil sections, a dilution of 1:80 may be used. This antibody was used to stain C2C12 cell line derived from mouse myoblasts at a dilution of 1:100

Anti-Human Polyvalent Immunoglobulins (whole molecule)−FITC antibody produced in rabbit has been used in immunohistochemical analysis and immunofluorescence

Biochem/physiol Actions

IgG (immunoglobulin G) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgG antibody participates in complement fixation and opsonization.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)