PORTHOS

93122323, Porcine thyroid, Epithelial

• UNSPSC Code: 41106514

Properties

• Product Name: PORTHOS, 93122323
• biological source: Porcine thyroid
• packaging: tube of 5 μg 93122323-DNA-5UG; pkg of vial of cells 93122323-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Thyroglobulin, plasminogen activator (u-PA)
• receptors: Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Porcine thyroid epithelial

Cell Line Description

Derived from porcine thyroid. The cells have been adapted to grow in low serum (0.1%) media.

Application

Regulation of thyroid cell metabolism

Culture Medium

Click:RPMI (1:1) + 10μg/ml Insulin + 10mM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 10mU/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 seeding at 2 x 10 ⁴ cells/cm² using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO₂; 37°C.

Other Notes

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