Product 90071810 includes two types of items. The DNA-5UG is 5 micrograms of purified DNA from this cell line. The -1VL is the cryopreserved cell line. Each vial contains 2-4 x 10,000 vialble cells that are ready for culture upon thaw.
PC-9 (formerly known as PC-14)
90071810, human lung, Heterogeneous mixture of round cells and spindle shaped cells
Synonym(s):
PC-9/S1 Cells, PC-9S1 Cells, PC9 Cells
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About This Item
UNSPSC Code:
41106514
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Product Name
PC-9 (formerly known as PC-14), 90071810
biological source
human lung
growth mode
Adherent/suspension
karyotype
Not specified
morphology
Heterogeneous mixture of round cells and spindle shaped cells
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human adenocarcinoma
Cell Line Description
Derived from a human adenocarcinoma from lung tissue which remains undifferentiated.
PC-14 was originally deposited with the Riken BioResource Center as a cell line derived from human lung adenocarcinoma (undifferentiated type) in 1989. The RIKEN BioResource Center subsequently informed the European Collection of Cell Cultures (ECACC) that short tandem repeat (STR) DNA profile analysis, undertaken in collaboration Immuno-Biological Laboratories Co., Ltd Japan, has shown this cell line to be identical to PC-9 a cell line derived from human lung adenocarcinoma (differentiated type). The misidentification occurred prior to the cell line being deposited at the RIKEN BioResource Center. The name of the cell line has been changed to PC-9 to reflect this finding.
PC-14 was originally deposited with the Riken BioResource Center as a cell line derived from human lung adenocarcinoma (undifferentiated type) in 1989. The RIKEN BioResource Center subsequently informed the European Collection of Cell Cultures (ECACC) that short tandem repeat (STR) DNA profile analysis, undertaken in collaboration Immuno-Biological Laboratories Co., Ltd Japan, has shown this cell line to be identical to PC-9 a cell line derived from human lung adenocarcinoma (differentiated type). The misidentification occurred prior to the cell line being deposited at the RIKEN BioResource Center. The name of the cell line has been changed to PC-9 to reflect this finding.
Application
PC-9 cell line has been used to study the effects of α-tocopherol addition on various cytotoxic drugs.[1]
Tumourigenicity studies
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8
D16S539: 9
D5S818: 11
D7S820: 10
THO1: 7
TPOX: 11
vWA: 17
CSF1PO: 11
D13S317: 8
D16S539: 9
D5S818: 11
D7S820: 10
THO1: 7
TPOX: 11
vWA: 17
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Seed cells at 2-4 x 10,000 cells/cm2; 5% CO2; 37°C. Remove attached cells by brief treatment with 0.25% trypsin or trypsin/EDTA.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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Articles
DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
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You seem to be selling 5ug of DNA from those cells. Please confirm that cells will be viable and can be used for cell culture. I assume 5ug of DNA is just the equivalent amount to the number of cells you are shipping? Thank you!
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What is the size of these cells?
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This is a product of the ECACC. The supplier does not report a size range for this cell line, but provides representative images that may be observed below.
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